摘要
目的观察不同碘水平对仔鼠大脑皮质发育的形态学影响。方法断乳后1个月大鼠饮用不同浓度的碘水,饲养3个月后雌雄合笼,取第2代60日龄仔鼠,测量血清甲状腺激素值,测量全脑重量,观察大脑皮质内锥体细胞层锥体细胞的形态学改变(苏木精-伊红染色和镀银染色)。结果各碘过量组甲状腺激素水平呈下降趋势,100HI组呈明显甲低状态;各高碘组仔鼠大脑皮质神经元排列分层及细胞形态与适碘组相比,未见明显改变(P<0.05)。结论大鼠对碘摄入量的增高有较强的耐受性,本实验各碘过量组中均未见仔鼠大脑皮质锥体细胞产生明显的形态改变。但过高碘摄入时(100HI组)表现为甲状腺功能低下,是否会引起仔鼠大脑皮质神经元的功能改变,还有待于进一步实验研究。
Objective To observe the morphological changes of cerebral cortex of filial generation of rats after fed with different doses of KI. Methods Seveaty Wistar rats were divided into five groups by randomized design. The rots were given drinking water including different iodine doses. After being fed three months, the rats were mated and their filial generations at the age of 60 day were observed. The serum thyroid hormones were meathead. The whole brains were weighted. The pyramidal celia in internal pyramidal layer of cerebral cortex were also observed by HE staining and silver impregnation technique. Results The serum thyroid hormones were gradually decreased in iodine excess groups and were significantly hypothyroidism in 100 HI group. Compared with NI group, there were no significant differenr of the pyramidal celia in internal pyramidal layer of cerebral cortex in all iodine excess groups （P〉0.05）. Conclusion Wistar rots have high endurance to high doses of iodine. There were no significant morphological changes of the pyramidal cells in internal pyramidal layer of cerebral cortex in all iodine excess groups. However, the serum thyroid hormones were decreased obviously in 100HI group,even appeared as hypothyroidism. It was still a question that if iodine excess can result in the functional alterations of pyramidal ceils of cerebral cortex.
出处
《解剖学研究》
CAS
2006年第3期190-192,i0002,共4页
Anatomy Research
基金
国家自然科学基金重点资助项目(30230330)
关键词
碘过量
仔鼠
大脑皮质
锥体细胞
Iodine excess
Filial generation
Cerebral cortex
Pyramidal cell
