摘要
本文用荧光光谱、紫外-可见吸收光谱法和循环伏安法研究了稀土金属离子Eu髥、Pr髥与人血清白蛋白(HSA)的相互作用。实验发现:Eu髥和Pr髥对HSA有较强的荧光猝灭作用。用Stern-Volmer方程分别对实验数据进行处理,结果发现:HSA与Eu髥、Pr髥发生反应生成了新的复合物,发生了分子内的非辐射能量转移。Eu髥、Pr髥对HAS的荧光猝灭作用,属于静态荧光猝灭。荧光猝灭图表明:Eu3+和Pr3+在HSA分子中至少有两类结合位点,Eu3+与HSA形成2.76∶1的复合物,结合常数lgK分别为12.03和9.05;Pr3+与HSA形成2.2∶1的复合物,结合常数lgK分别为9.89和6.97。同时用圆二色谱及同步荧光光谱法探讨了Eu髥和Pr髥对HSA构象的影响。
The interaction of Eu（Ⅲ） and Pr（Ⅲ） with HSA was studied by fluorescence spectrum and ultra-violet spectrum and electrochemistry. It was shown that these ions have a quite strong ability to quench the fluorescence launching of HSA. After analyzing the fluorescence quenching data according to Stern-Volmer equation, It was found that HSA reacted with Eu（Ⅲ） and Pr（Ⅲ） to form a new complex-system. The quenching belonged to static fluorescence quenching, with non-radiation energy transfer happening within single molecule, The composition of the Eu-HSA complex is Eu2.76：HSA and the binding constants： lgK=12.03, lgK=9.05; The composition of the Pr-HSA complex is Pr2.2：HSA and the binding constants： lgK=9.89, lgK=6.97 at room temperature. The effect of Eu （Ⅲ） and Pr（Ⅲ） on the conformation of HSA was analyzed by circular dichroism （CD） and synchronous fluorescence spectra.
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
2006年第9期1615-1622,共8页
Chinese Journal of Inorganic Chemistry
基金
甘肃省自然科学基金(No.3ZS051-A25-021)
甘肃省高分子材料重点实验室资助项目。
关键词
稀土离子
人血清白蛋白
吸收光谱
荧光光谱
圆二色谱
电化学
rare earth ions
human serum albumin
absorption spectra
fluorescence spectra
circular dichroism spectra
electrochemistry
