摘要
利用发根农杆菌A4,A4 9615,1.2556诱导中药黄芩外植体,得到黄芩不定根,根据形态和在MS培养基中进行培养证实得到黄芩毛状根,经抗菌素氨苄西林钠500 mg/L除菌后,毛状根表现出分枝多,生长快,且能在MS中生长繁殖的特性,为采用生物技术生产黄芩活性成分奠定基础。
Hairy roots were induced from Scutellaria baicalensis Georgi by three kinds of Agrobacteriurn rhizogenes strains A4, A4 9615 and 1. 2556. They were revealed by appearance and growing on hormone-free medium. There are some significant characteristics of hairy roots after eliminating bacteria with antibiotic, such as more branches, fast growth capacity and growth in hormone-free media. It is the basis for producing active components of Scutellaria baicalensis Georgi by biotechnology.
出处
《西北农业学报》
CAS
CSCD
北大核心
2006年第5期244-247,共4页
Acta Agriculturae Boreali-occidentalis Sinica
关键词
黄芩
发根农杆菌
毛状根
诱导
Scutellaria baicalensis Georgi
Agrobacterium rhizogenes
Hairy roots
Induct
