摘要
在pH4．56的Britton—Roblnson（B-R）缓冲溶液中，半胱氨酸的-SH和-NH3^＋分别与金纳米粒子表面进行共价结合和静电作用，导致金纳米粒子的长距离自组装，形成网状超分子结构，并使金纳米粒子的最大吸收波长从520nm红移到660nm。本实验对半胱氨酸引导的金纳米粒子自组装的作用机制进行了研究，建立了操作简便、高灵敏度测定半胱氨酸的分析方法。其线性范围为0．01—0．20mg／L；检出限为2．8μg/L（3σ，2．3×10^-8mol／L）。在实验条件下，其它常见的氨基酸和谷胱甘肽均不干扰测定。
Due to the covalent combination with the SH group and the electrostatic binding with the NH3^＋ group of cysteine, gold nanoparticles can self-assemble and form a supermolecular network structure in the Britton-Robinson buffer solution （ pH 4.56）. As a result, absorption peak of the gold nanoparticles shifts from 520 nm to 660 nm. The mechanism of self-assembly of gold nanoparticles directed by cysteine was investigated. Based on the study, a simple and sensitive method for cysteine determination has been estab- lished. There is a good linear relationship between the absorbance at 660 nm and cysteine concentration in the range of 0.01 -0.20 mg/L. The corresponding detection limit is 2.8 μg/L （3σ,2.3×10^-8mol/L）. Common amino acids and glutathione do not interfere with the determination.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2006年第8期1149-1152,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.20375011)
河北省自然科学基金(No.203111)资助项目
