摘要
RNA干扰作用(RNA i)是通过双链RNA介导的特异性转录后基因沉默,这一过程已在拟南芥、线虫和真菌等多种模式生物中得到提示。RNA i主要通过双链RNA被核酸酶切割成的小干扰RNA(siRNA),由siRNA介导识别并靶向切割同源靶mRNA分子而实现的。RNA i具有高效性和高特异性,已成为关闭基因的一项新技术,在基因功能研究和疾病的基因治疗中具有广阔的应用前景。
RNA interference（RNAi） is something of post- transcriptional gene silencing,specifically mediated by double - stranded RNA. RNAi was observed in many model organisms, such as Arabidopsis, C. elegans and fungi. The basicprocess in RNAi involvesa dsRNA that is processed by endonuclease into small interference RNA（siRNA） that guides recognition and targeted cleavage of homologous target mRNA. Being effective and highly specific, RNAi has becames a novel technique in knocking gene down plays important roles in gene function study and gene therapy diseases.
出处
《河南科技学院学报》
2006年第1期5-8,共4页
Journal of Henan Institute of Science and Technology(Natural Science Edition)
