摘要
目的 提取花椒挥发油，并对其进行抗人肺癌杀伤及诱导凋亡试验。观察花椒是否具有抗肿瘤活性，并确定其最小杀伤肿瘤细胞的剂量；同时观察诱导细胞凋亡的最小剂量以确定其抗肿瘤机制。方法 利用改进水蒸气法提取花椒挥发油，利用Tween-80作为助溶剂对花椒倍比稀释，对人A549肺癌细胞进行抗肿瘤试验，利用SRB方法和形态学确认细胞杀伤率，采用荧光染料Hochest33258染色检测细胞的凋亡水平。将试验同时分为时间-效应组（24、48、72h）和剂量-效应组（0．125～16mg／ml）。结果 ≥4mg／ml以上剂量的花椒挥发油提取物对A549具有较高的抑瘤率，达到87．9％；最低抑瘤浓度为1mg／ml，抑瘤率为40．2％。形态学观察结果表明，被杀伤的细胞体积萎缩，细胞膜皱缩，成活细胞数稀疏，数量明显低于空白对照组。不同给药时间的研究结果表明，药物最佳作用时间为48h及72h。利用低于杀伤浓度的剂量作用于A549进行研究，观察其对A549细胞凋亡的影响，利用Hochest33258观察对细胞核的影响。结果表明，0．51mg／ml剂量的花椒挥发油提取物作用于细胞72h对肿瘤细胞具有明显的诱导细胞凋亡作用。结论 高浓度（4～16mg／m1）的花椒挥发油对人肺癌A549细胞株有杀伤作用，低浓度（1mg／ml）的花椒挥发油对A549有诱导细胞凋亡的作用。
Objective The essential oil of Zanthoxylunm Maxim. Was isolated and its anti- tumor and apoptosis inducing test were performed. The anti-tumor and its mechanism were checked by the time-dependent and dose-dependent. The minimal agents of anti-tumor and apoptosis was assayed. Methods The improved steam distillated was used to isolated the essential oil of Zanthoxylunm Maxim. Tween-80 was used to dissolve the essential oil and diluted by saline. The Human lung cancer cell line A549 was applied in this study. The anti- tumor effect was assayed by the SRB method and the apoptosis inducing effect was assayed by Hochest33258 stain. The experiment groups were divided into time-dependent （24,48, 72h） and dose-dependent （0.25～16mg/ml）. Results Equal or above 4mg/ml dose of essential oil of Zanthoxylunm Maxim could markedly inhibit the growth of A549. The highest inhibit rate was 87. 9%. The minimal inhibit rate was 40. 2% and the minimal inhibit dose was 1mg/ml. The dose between 0. 25mg/ml and 1mg/ml has the apoptosis inducing effect on A549. Conclusion The higher concentration （4～ 16mg/ml） of essential oil of Zanthoxylunm Maxim. has the anti-tumor effect on human lung cancer cell line A549. The lower concentration （1mg/ml） of essential oil of Zanthoxylunm Maxim. has the apoptosis inducing effect on human lung cancer cell line A549.
出处
《蛇志》
2006年第3期183-186,共4页
Journal of Snake
基金
国家自然科学基金资助课题(编号:30572177)。
关键词
花椒
抗肿瘤
机制
Zanthoxylunm Maxim
Anti-tumor
mechanism
