摘要
目的研究泼尼松(Predn isone,PRD)对大鼠实验性变态反应性脑脊髓炎(EAE)的治疗作用和作用机制。方法豚鼠脊髓匀浆和完全福氏佐剂制成的混合液诱导Lew is大鼠建立EAE模型,10 d后将PRD 5 mg/kg灌胃给予EAE大鼠,连续给药16 d,观察其发病情况、外周血T淋巴细胞CD3+、CD4+、CD8+、NK细胞比率以及脑、脊髓组织病理变化。结果模型组CD3+T淋巴细胞比率63.2%,CD4+T淋巴细胞比率46.8%,CD8+T淋巴细胞比率17.4%,NK细胞比率10.2%;泼尼松组CD3+T淋巴细胞比率69.5%,CD4+T淋巴细胞比率51.8%,CD8+T淋巴细胞比率18.1%,NK细胞比率5.3%。PRD可显著改善恢复期EAE的临床评分,降低外周血NK细胞比率,升高外周血T淋巴细胞CD3+、CD4+比率,抑制大鼠大脑的炎症反应和脱髓鞘改变,但对外周血T淋巴细胞CD8+、CD4+/CD8+比率等无明显影响。结论PRD对EAE大鼠具有治疗作用,其作用机制可能与降低外周血NK细胞比率、升高T淋巴细胞CD3+和CD4+比率有关。
Objective To investigate the effects of prednisone （PRD） on experimental allergic encephalomyelitis （EAE） and the mechanism. Methods Lewis rats were injected with mixture of guinea pig spinal extracts and Freund＇s adjuvant （FA） to induce EAE. PRD 5 mg/kg was orally given ten days after the injection. The clinical manifestation of rats, their peripheral blood lymphocytes CD3^ ＋ , CD4^ ＋ , CD8^ ＋ and NK subsets, and pathology changes of brain and spinal were studied on day 26. Results In model CD3^ ＋ ratio is 63.2% , CD4 ^＋ ratio 46. 8%, CD8 ^＋ratio 17.4% and NK ratio 10. 2%. In PRD CD3 ^＋ ratio is 69. 5%, CD4^＋ ratio 51.8%, CD8^ ＋ ratio 18. 1% and NK ratio 5.3%. PRD obviously decreased the clinical scores of EAE rats and peripheral blood NK ratio, increased CD3 ^＋ and CD4^ ＋ ratio, inhibited brain inflammatory reaction and demyelination. However, PRD didn＇ t affect peripheral blood lymphocytes CD8 ^＋ and CD4^ ＋/CD8^ ＋ ratio. Conclusions PRD is effective in the treatment of EAE rats, and the therapeutic mechanism may be contributed to the decrease of the peripheral blood NK ratio and increase CD3 ^＋ and CD4^ ＋ ratio.
出处
《基础医学与临床》
CSCD
北大核心
2006年第8期877-881,共5页
Basic and Clinical Medicine
基金
北京市自然科学金(7062022)
北京市中医药科技发展资金(2003Ⅲ-21)
关键词
多发性硬化
实验性变态反应性脑脊髓炎
大鼠
multiple sclerosis
experimental allergic encephalomyelitis
prednisone
rat
