摘要
通过单因素实验和正交实验,对MHL4.07菌摇瓶发酵产脂肪酶的培养基组成和培养条件进行了研究,结果表明:最佳发酵培养基配方为橄榄油15.0g/L、玉米浆40.0g/L、NH4Cl20.0g/L、豆饼粉20.0g/L、MgSO40.5g/L、KH2PO410.0g/L、KCl1.0g/L。最佳发酵条件为种子培养84h、接种量7%、初始pH6.5、装液量50mL/250mL三角瓶,培养温度28℃,摇床转速160r/min,发酵培养96h。在此条件下,最高脂肪酶产量达到60.54U/mL,比优化前提高了44.5%。
The influences of fermentation conditions on lipase produced by Rhizopus arrhizus were investigated through the single factor and the orthogonal experiments. The results show that： The compositions of optimal fermentation medium were olive oil 15.0g/L, corn steep liquor 40.0g/L, NH4Cl 20.0g/L, bean cake flour 20.0g/L, MgSO4 0.5g/L, KH2PO4 10.0g/L, KCl 1.0g/L. The optimum conditions were： the culture time of seed 84h, seed volume 7%, the initial pH 6.5, 50mL in 250mL total volume, the temperature of the shaking table 28%, rotating speed 160r/min, the fermentation time 96h. The maximum yield of lipase was 60.54U/mL and it was increased 44.5% under the optimum conditions.
出处
《食品科技》
CAS
北大核心
2006年第8期39-42,共4页
Food Science and Technology
关键词
根霉
脂肪酶
发酵条件
正交实验
lipase
rhizopus arrhizus
fermentation conditions
orthogonal experiments
