摘要
本文研究了菹草类胡萝卜素提取物(CEPC)对人宫颈癌Hela细胞增殖和凋亡的影响,采用体外培养技术,用可见光显微镜、荧光倒置显微镜、透射电镜和激光扫描共聚焦显微镜(LSCM)观察Hela细胞的形态变化。结果表明:分别用浓度为2.5、5、10μg/ml的CEPC处理Hela细胞,在可见光、荧光显微镜、电镜和LSCM下观察细胞形态,分别出现了细胞数量明显减少、皱缩变形、体积缩小、触角伸长,显示亮黄色荧光的细胞核呈现“新月状”、条状甚至碎片状,细胞膜表面绒毛减少,胞浆内空泡明显增多,细胞核染色体发生固缩,细胞中RNA的含量明显增加等典型的凋亡细胞形态特征。实验结果表明CEPC对Hela细胞的增殖具有明显的抑制作用并能有效地诱导Hela细胞凋亡。
The effects of carotenoids extracts from Potamogoton crispus L. （CEPC） on proliferation and apoptosis of Hela cells were studied. This paper adopted visible light, fluorescence, electron microscopy and Laser Scanning Confocal Microscope （LSCM） to observe the cellular morphology of Hela cells under the effecfs of CEPC. After cells were treated with 2.5, 5, 10μg/ml CEPC, respectively, many typical apoptotic morphological features were observed by these apparatus, such as： the number of cells decreased, the cells shrunk, the cells distorted, the volume reduced, the antennas prolonged, the shape of cell nucleuses appeared like crescent or piece, the villus of cell membrane surface reduced, the vacuole inside plasm obviously increased, the chromosome in the cell nucleus shrunk and the content of RNA in the cell increased. So it suggested that the carotenoids of Potamogoton crispus L. have a marked inhibition on the proliferation of Hela ceils and can induce Hela ceils apoptosis.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第8期240-244,共5页
Food Science
基金
教育部2002年度博士点基金资助项目(20020504008)
湖北省科技攻关项目(2003AA20412307)
