摘要
目的探讨不同剂量维生素 C(vitamin C,VC)对脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)诱导的体外培养人外周血单个核细胞(human peripheral blood mononuclear cells,HPBMC)HLA-Ⅰ表达抑制作用的影响。方法采用流式细胞术(flow cytometry,FCM)、免疫细胞化学和蛋白印迹(Western blotting)方法研究了不同剂量(25μmol/L 和100μmol/L)VC 预处理对 DON 诱导的 HPBMCHLA-Ⅰ表达抑制作用的影响。结果 FCM 定量检测结果表明,DON 处理组 HPBMC HLA-Ⅰ的表达与正常对照组相比明显降低(FI 值0.88±0.02比1.00±0.03,P<0.05)。25μmol/L 和100μmol/LVC 预处理+DON 组 HPBMC HLA-Ⅰ表达的 FI 值分别为1.15±0.06和1.10±0.02,均明显高于DON 处理组(0.88±0.02,P<0.05)。同时,25μmol/L 和100μmol/L VC 单独处理组 HPBMC HLA-Ⅰ的表达量也均明显高于对照组(FI 值1.28±0.03和1.25±0.05比1.00±0.03),表明 VC 可在一定程度上促进体外培养的 HPBMC HLA-Ⅰ表达(P<0.05)。免疫细胞化学结果表明,与 DON 处理组相比,两种不同剂量 VC 预处理+DON 组 HLA-Ⅰ表达阳性的细胞百分率比 DON 处理组明显增高(70.10%±6.90%和64.50%±5.50%比42.20%±4.30%,P<0.05)。蛋白印迹同样证实了以上结果。结论 VC 可一定程度的拮抗 DON 对体外培养的 HPBMC HLA-Ⅰ表达的抑制作用。
Objective To explore the putative effects of Vitamin C （Vit C） on inhibition of human leucocyte antigen class Ⅰ （HLA-Ⅰ） expression of human peripheral blood mononuclear cells （HPBMCs） induced by deoxynivalenol （DON） in vitro. Methods The effects of Vit C on the changes of HLA-Ⅰ expression of HPBMCs induced by DON in vitro were evaluated with cell culture, flow cytometry （ FCM）, Western blotting and immunocytochemical methods. Results FCM analysis showed that HLA-Ⅰ expression of HPBMCs in DON treated cells was significantly lower than that in controls （ FI 0. 88 ± 0. 02 vs 1.00 ± 0. 03, P 〈 0. 05）. As compared with DON group, the HLA-Ⅰ expressions of HPBMCs in the two Vit C （25μmol/L and 100 μmol/L） pretreatment groups were all significantly increased （ 1.15 ± 0. 06 and 1.10 ± 0. 02 vs 0. 88 ＋ 0. 02, P 〈 0. 05 ）. Exposure to different dosage of Vit C alone could dramatically increase the expression of HLA-Ⅰ of HPBMCs in vitro as compared with that in the normal control （ FI for 25μmol/L and 100μmol/L Vit C treatment group was 1.28 ± 0.03 and 1.25 ± 0.05 respectively, P 〈 0.05）. Immunocytochemical results showed that the percentages of HLA-Ⅰ positive expression of HPBMCs in Vit C pretreatment groups at different dosages were significantly higher than those in DON group （70. 10 ± 6. 90） %, （64. 50 ± 5. 50） % vs （ 42. 20 ± 4. 30 ） %, P 〈 0. 05. Western blotting confirmed the results of FCM and immunocytochemistry. Conclusion Vitamin C pretreatment at different dosages could reverse at some extent the inhibitive effects of DON on HLA-Ⅰ expression of HPBMCs.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2006年第5期314-318,F0003,共6页
Chinese Journal of Preventive Medicine
基金
国家科技部基础研究重大项目前期研究专项资助项目(2001ccc00500)
河北省自然科学基金(C2005000763)
