摘要
目的：建立测定人血浆中利多卡因、布比卡因浓度的高效液相色谱方法。方法：色谱柱Waters Spherisorb C8（4．6mm×250mm，5μm），流动相乙腈-1／15mol·L^-1磷酸盐缓冲液（21：79,v／v）．紫外检测波长230nm，流速1．0mL·min^-1，柱温：30℃。结果：利多卡因在0．112～2．973mg·L^-1范围内，浓度与峰面积之比线性关系良好（r=0．9998）．最低检测限为2ng，平均回收率为100．78%～102．65％，日内、日间RSD分别小于4．7%和6．7％。布比卡因在0．102～2．555mg·L^-1范围内．浓度与峰面积之比线性关系良好（r=0．9987），最低检测限为2ng，平均回收率为100．73％～102．111％，日内、日间RSD分别小于5．8%和7．3％。结论：本方法适用于利多卡因、布比卡因的临床药动学研究。
OBJECTIVE An HPLC method was established for determination of lidocaine and bupivacaine in children＇s plasma. METHODS The plasma sample was separated on Waters Spherisorh C8 （4. 6 mm × 250 mm, 5μm） column with a mobile phase of a mixture of acetontrile and phosphate buffer（21：79,v/v）, the temperature was set at 30℃ with flow rate of 1.0 mL· min^-1 ,detection-UV at 230 nm. RESULTS A lmearity was obtained from 0. 112mg·L^-1 to 2. 973mg·L^-1 of lidocaine in plasma with a good correlation （r= 0. 999 8）. Detection limit was 2 ng. Average recoveries was 100. 78% - 102. 65%. Withinday RSD less than 4. 7% ,inter-day RSD less than 6. 7%. A linearity was obtained from 0.102 mg·L^-1 to 2. 555 mg·L^-1 of bupivacaine in plasma with a good correlation （r = 0. 998 7）. Detection limit was 2 ng. Average recoveries was 101. 73% - 102. 01%. Within-day RSD less than 0. 48%,inter-day RSD less than 1.15%. CONCLUSION This method is suitable for the study on pharmacokinetics of lidocaine and bupivacaine.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2006年第9期1075-1077,共3页
Chinese Journal of Hospital Pharmacy
