摘要
以pVAX1为真核表达载体,以猪白介素18基因、猪2型圆环病毒内蒙古株的衣壳蛋白和复制蛋白基因为目的基因,构建了pVAX1-ORF2、pVAX1-IL18-ORF2、pVAX1-ORF2-ORF1和pVAX1-IL18-ORF2-ORF1等4个重组质粒作为核酸疫苗。将这4个重组核酸质粒分别酶切鉴定后,转染BHK-21和PK-15细胞进行RT-PCR、间接免疫荧光和Western blotting检测。结果表明,重组核酸质粒构建正确,并能进行表达。
Using pVAX1 as eukaryotic expressing vector,swine interkine 18 genes,PCV2（Innermongolia strain） capsid and replicated protein genes as aimed genes, 4 recombinant plasmids were contructed as nucleotide vaccines. They are named as pVAX1-ORF2, pVAX1-ORF2-ORF1, pVAX1-IL18-ORF2, pVAXI-IL18-ORF2-ORF1. The four recombinant plasmids were transfected into BHK-21 cell after enzyme-identificated,and detected by RT-PCR,indirect immunofluorescence and Western blotting. The result shows that the nucleotide vaccines were constructed correctly and expressed in cells.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2006年第5期465-467,共3页
Chinese Journal of Veterinary Science
基金
国家"863"计划资助项目(2001AA213071-4)
