摘要
建立反相高效液相色谱-光电二极管阵列检测器法测定了不同品质栀子生品中栀子苷的含量。采用Nucleodur C18色谱柱（4．6mm×250mm,5μm），以甲醇-水（22：78，体积比）为流动相，流速1．0ml／min，检测波长240nm，柱温28℃。栀子苷在0．80～7．20懈范围内呈良好的线性关系（r=0．99998），加样回收率为99．3％。该方法简便、快速、准确，可用于栀子生品的质量控制。
HPLC method coupled with photodiode army detection（PAD） for the determination of geniposide in Fructus Gardeniae of different quality was established.The separation was achieved on a Nucleodur C18 column（4.6 mm × 250 mm, 5/μm） at 28 ℃ through the application of the melhanol-water（22 ：78）as the mobile phase at a flow rate of 1.0 ml/min and 240 nm as the detection wavelength. Geniposide showed good relationship at the range of 0.80 - 7.20 μg（ r = 0.999 98）. The recovery was 99.3 %. The proposed method was simple, rapid and accurate and promised to be applicable for the quality control of Fructus Gardeniae
出处
《安徽农业科学》
CAS
北大核心
2006年第17期4207-4208,共2页
Journal of Anhui Agricultural Sciences
