摘要
从健康成年人的口腔中筛选出一株产抑菌物质的菌株HO-69，经API系统鉴定为植物乳杆菌。排除酸性末端产物与过氧化氢的影响后，HO-69的发酵上清液对变形链球菌等革兰氏阳性菌、大肠杆菌等革兰氏阴性菌具有抑制作用。抑菌物质经初步提取，推测其分子量在10kDa以下，胰蛋白酶处理后抑菌活性部分损失，100℃水浴加热20min依然保持较高的抑菌活力，显示活性的pH范围是3．0～7．0，活力随pH的降低而增强。
HO-69, a strain producing antibacterial substance, was isolated from healthy oral cavity, and was identified by API system as Lactobacillus plantarum. The supernatant of HO-69 exhibited inhibitory activty against gram-positive and gram-negative bacteria including Streptococcus mutants and Escherichia coli, eliminated the effects of organic acids and hydrogen peroxide. The antibacterial substance had a low molecular weight less than 10kDa. It was sensitive to pepsin, and was heat stable. It was active within pH range from 3 to 7, and it showed higher activity at lower pH.
出处
《工业微生物》
CAS
CSCD
北大核心
2006年第3期13-17,共5页
Industrial Microbiology
关键词
植物乳杆菌
抑菌物质
筛选
性质
鉴定
Lactobadllus plantarum
antibacterial substance
screen
identification
characterization
