摘要
目的观察支气管哮喘(哮喘)患者外周血单个核细胞(PBMCs)中CD4+CD2+5调节性T细胞(Treg)水平及叉状头/翅膀状螺旋转录因子(Foxp3)mRNA表达的变化,探讨CD4+CD2+5Treg在哮喘发病中的作用。方法采用流式细胞仪检测78例哮喘患者(急性发作期组30例,慢性持续期组25例,缓解期组23例)和29例健康志愿者(正常对照组)PBMCs中CD4+CD2+5Treg的比例;反转录-聚合酶链反应(RT-PCR)检测PBMCs中Foxp3mRNA的表达。结果急性发作期组和慢性持续期组PBMCs中CD4+CD2+5Treg的比例及Foxp3mRNA的表达明显低于缓解期组和正常对照组(P<0.05);缓解期组CD4+CD2+5Treg的比例及Foxp3mRNA的表达虽亦低于正常对照组,但差异无统计学意义(P>0.05);急性发作期组CD4+CD2+5Treg的比例及Foxp3mRNA的表达低于慢性持续期组(P<0.05)。结论哮喘患者外周血中具有免疫抑制活性的CD4+CD2+5Treg数量减少,功能下降,可能参与哮喘的发生和发展。
Objective To investigate changes of CD4^＋CD25^＋ regulatory T cells （CD4^＋CD25^＋ Treg） and forkhead/winged helix transcription factor （Foxp3） mRNA in peripheral blood mononuclear cells （PBMCs） from patients with asthma, so as to elucidate the possible roles of CD4^＋CD25^＋ Treg in the development of asthma. Methods The peripheral blood samples were collected from 29 healthy controls （normal control group） and 78 patients with asthma which included 30 patients in exacerbation grunp, 25 patients in persistent group, and 23 patients in remission group. By using flow cytometry and RT - PCR, the CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA in PBMCs were detected. Results The CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA in PBMCs of exacerbation and persistent group were lower than that of remission grollp and normal control group （P 〈0. 05 ）. Although the CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA of remission group were also lower than that of normal control group, but there were no significant difference between them （ P 〉0. 05 ）. Compared with persistent group, exacerbatioll group had lower CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA （P 〈0. 05）. Conclusion The decrease of CD4^＋CD25^＋ Treg ratio and its function in PBMCs may be responsible for pathogenesis of asthma.
出处
《中国医师进修杂志（内科版）》
2006年第9期11-13,共3页
Chinese Journal of Postgraduates of Medicine
基金
湖北省科技攻关项目(2003AA301C10)
