摘要
目的探讨复苏后缺血缺氧性脑病大鼠脑组织含水量(BWC)与水通道蛋白-4(aquaporin-4,AQP4)表达水平的动态变化及其相关性。方法建立大鼠窒息心肺复苏模型,干湿质量法测定自主循环恢复后1、3和6 h BWC的变化;同时,利用半定量免疫组织化学的方法检测脑组织AQP4表达水平的变化;分析两者的相关性。结果大鼠复苏后,随时间的延长BWC明显增加。各时间点BWC与对照组相比均有显著性差异(P值均<0.05)。其中,复苏组大鼠6 h BWC明显高于1、3 h;而1、3 h大鼠的BWC无明显差异。同时,复苏后脑组织AQP4水平的表达,也呈现出与BWC变化几乎相同的趋势。相关分析表明,BWC与脑组织AQP4呈明显正相关(r=0.791,P<0.01)。结论复苏后早期大鼠已出现明显的脑水肿,且与脑组织AQP4表达水平正相关,提示AQP4可能参与复苏后早期脑水肿的形成过程。
Objective To study the dynamic change and the correlation of brain water content （BWC） with AQP4 expression level in hypoxia ischemic encephalopathy in rats after CPR. Methods The cardiac arrest model of rat was duplicated by asphyxy method. The BWC was measured by means of wet - to - dry weight ratio at 1,3 and 6 h after ROSC, and the AQP4 expression level in brain tissue was assessed by semi -quantization immuno- histochemistry staining at the same time. The correlation was analyzed between the BWC and AQP4 expression level . Results The BWC of rats increased obviously along with the prolongation of time fol- lowing ROSC . The brain water contents at 1,3 and 6 h after ROSC were significantly higher than those of the control group （ P 〈 0.05）. Moreover, the BWC at 6h after ROSC was much higher than those at 1 and 3 h after ROSC ; and there was no significant difference in the BWC between the groups at 1 h and 3 h after ROSC. At the same time, the change of AQP4 expression level in brain tissue was as nearly same as that of the BWC. The correlation analysis demonstrated further that the BWC was correlated apparently with the AQP4 expression level in brain tissue （ r = 0. 791, P = 0. 000 ）. Conclusion The brain edema has formed obviously in the early stage after ROSC , and it is positively correlated with the AQP4 expression level in brain tissue, indicating that AQP4 participates probably in the development of brain edema.
出处
《中国急救医学》
CAS
CSCD
北大核心
2006年第9期673-674,共2页
Chinese Journal of Critical Care Medicine
基金
江苏省教育厅"211"工程资助项目(No.R2317054)
关键词
心肺复苏
脑组织含水量
水通道蛋白-4
Cardio - pulmonary resuscitation
Brain water content
Aquaporin - 4
