摘要
目的探讨河南高发区青年食管癌和癌前病变组织中STAT3的表达及意义,加深对食管癌变机理的了解。方法采用免疫组化卵白素—生物素—过氧化物酶复合物(ABC)法和组织病理学方法,分析STAT3在36例食管癌和癌旁组织中的变化特征和规律。结果从正常食管上皮→基底细胞过度增生→间变→至鳞状细胞癌的演变过程中,随着病变程度的加重,STAT3蛋白阳性表达率呈现下降趋势,分别为86%(12/14)、88%(28/32)、55%(11/20)和75%(27/36),其中基底细胞过度增生与间变(P=0.008)的阳性率差异具有统计学意义。STAT3蛋白在高分化鳞癌组织中的阳性率最高89%(16/18),中、低分化鳞癌组织阳性率逐渐下降,分别为60%(6/10)和63%(5/8),但差异无统计学意义。结论STAT3蛋白在癌变早期阶段表达变化显著,并随癌变程度加重和癌组织分化程度下降而呈现阳性表达率下降的趋势,提示STAT3是食管癌变早期的重要分子事件,并可能是食管癌变重要分子基础之一。
Objective To characterize the expressions and significance of STAT3 in esophageal precancerous and cancerous lesions from the young adults at a highincidence area of esophageal cancer in Henan Province, To further comprehend the mechanism of esophageal carcinogenesis. Methods STAT3 expression was detected by immunohistochemical method （ABC） in 36 SCC patients and precancerous lesions. Results With the lesions progressed from normal epithelium （NOR）→basal cell hyperplasia （BCH）→dysplasia （DYS）→esophageal squamous cell carcinoma （SCC）, a declining tendency of STAT3 expression was observed, the positive rates of STAT3 were 86% （ 12/14）, 88% （28/32）, 55% （11/20） and 75% （27/36）, The differences were significant （ P 〈 0.05） between BCH vs DYS. The positive rate of STAT3 in well differentiation SCC （89% , 16/18） was higher than moderate differentiation SCC （60 % , 6/10） and poor differentiation SCC （63 % , 5/8）, but the difference was not significant （ P 〉 0.05）. Conclusions STAT3 expressed remarkably at the early process of esophageal carcinogenesis. With the lesions progressed and the differentiation became poor, a declining tendency of STAT3 expression was observed. The present results demonstrated that STAT3 protein expression was an important molecular event in early process of esophageal carcinogenesis and it might be one of the important molecular basis of esophageal carcinogenesis in young EC.
出处
《肿瘤基础与临床》
2006年第5期353-355,共3页
journal of basic and clinical oncology
基金
国家杰出青年科学基金(编号:30025016)
河南省医学科技攻关项目(编号:20058)
