摘要
建立了用反相离子对高效液相色谱法测定人血浆中阿昔洛韦浓度的方法。色谱柱采用ＡｌｌｔｅｃｈＣ＿１８分析柱，以０．０４ｍｏｌ／Ｌ氯化钠－甲醇－ＩＰＲ－Ｂ７离子对色谱试剂混合液（１００：１５：０．６；ｖ／ｖ）为流动相。检测波长为２５４ｎｍ，肾上腺素为内标。血浆样品经高氯酸沉淀蛋白后直接进样测定。阿昔洛韦浓度在４０～１６００ｎｇ／ｍｌ范围内线性良好，ｒ＝０．９９９２．测定含阿苷洛韦１６０ｎｇ／ｍｌ的血浆样品，其日内（ｎ＝７）及日间的ＲＳＤ分别为４．７％和３．０％。平均回收率为９８．２±５．３％。测定了１０名健康志愿者单次口服阿昔洛韦片剂４００ｍｇ后不同时间的血药浓度并计算了有关的药代动力学参数。
A simple,rapid and accurate ion-pair RP- HPLC method was devloped for the determination of acyclovir in human plasma.The analytical column was packed with Alltech- C18. The mobile phase was 0.04 mol/L sodium chloride-methanol-IPR-B7 agent(100 : 15 : 0.6,v/v). The plasma sample was injected directly into the HPLC system after precipitating the proteins with perchloric acid.The compounds were quantitated using an ultraviolet detector operated at 254 nm which allowed the determination of 40～1600 ng/ml of acyclovir with reproducibility.The limit of detection was 20 ng/ml. Intra-day and inter-day RSD for assaying the plasma sample containing 160 ng/ml concentration of acyclovir was 4.7%(n=27) and 3.0%(n=7),respectively. The mean recovery was 98.2%. The pharmacokinetics ofacyclovir in ten healthy volunteers after oral administration of 400 mg was studied.
出处
《中国药科大学学报》
CSCD
北大核心
1996年第11期674-676,共3页
Journal of China Pharmaceutical University
