摘要
目的研究血管内皮生长因子受体-3(VEGFR-3)在人宫颈癌细胞凋亡过程中的作用。方法用基因重组方法构建人反义VEGFR-3基因真核表达载体,用电穿孔法转染人宫颈癌细胞系Hela细胞。采用WesternBlot分析转染前后细胞中VEGFR-3蛋白的变化。利用Hoechst33258染色和流式细胞仪观察转染后Hela细胞的凋亡情况。结果转染反义VEGFR-3质粒后,Hela细胞VEGFR-3的蛋白表达水平明显下降,细胞凋亡率明显增加(P<0.01)。结论抑制VEGFR-3的表达可促进宫颈癌细胞的凋亡,VEGFR-3可能是肿瘤基因治疗的一个潜在新靶点。
Objective To investigate the effect of vascular endothelial growth factor receptor-3 （VEGFR- 3） on the apoptosis of cervical cancer cells. Methods VEGFR-3 antisense gene eukaryotic expression vector was constructed and transfected to human cervical cancer cell line, Hela cell line by electroporation. The expression level of VEGFR-3 protein before and after transfection was determined by Western Blot and the apoptosis of Hela cell line was observed by Hoechst33258 and flow cytometry. Results After the transfection of the antisense VEGFR-3 plasmid, the expression level of VEGFR-3 protein decreased significantly, and the apoptotic rate of Hela cells increased, compared with the control（P〈 0.01）. Conclusion Antisense VEGFR-3 transfection may induce the apoptosis of the human cervical cancer cells. Thus VEGFR-3 might be a potential target for gene therapy.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2006年第9期659-661,共3页
Cancer Research on Prevention and Treatment
基金
国家重点基础研究发展规划项目973(2002CB513107)
关键词
VEGFR-3
反义核酸
凋亡
宫颈癌
VEGFR-3
Antisense nucleic acid
Apoptosis
Cervical cancer
