摘要
目的:探讨核因子NF-κB激活在人宫颈鳞癌发生及发展中的作用。方法:125例不同组织学分级的人类宫颈标本为实验对象,包括32例轻度鳞状上皮非典型增生(LSILs/CIN-Ⅰ),33例重度鳞状上皮非典型增生(HSILs或CIN-Ⅱ/CIN-Ⅲ),38例鳞状细胞癌(SCCs),22例正常宫颈(normal)。SCCs中包括分化良好宫颈鳞癌(WDSCC)9例,中等程度分化(MDSCC)20例,分化较差(PDSCC)9例。应用免疫组织化学方法,免疫印迹方法检测NF-κBp65在不同组织学分级中的表达水平。结果:①免疫组织化学显示在正常宫颈组织及轻度宫颈上皮内非典型增生组织中,RelA主要定位于胞浆,而在重度宫颈上皮内非典型增生及宫颈鳞状细胞癌中,RelA主要位于胞核中。②免疫印记方法显示在正常宫颈组织及轻度宫颈上皮内非典型增生组织中RelA主要在胞浆提取物中,而在重度宫颈上皮内非典型增生及宫颈鳞状细胞癌中胞核提取物中表达增强,而在胞浆提取物中相对减少,差异显著(P<0.05)。结论:NF-κB活化与宫颈癌的发生及发展有关,NF-κBp65可能成为人类宫颈癌治疗的新靶点。
AIM： To evaluate the significance of NF-κB p65 protein expression in the development of human cervical squmous cell cancer. METHODS： Immunohistochemical analysis was done in 125 casas of paraffin -embedded cervical tissue specimens of different histological grades （32 LSILs, 33 HSILs, 38 SCCs and 22 normal） to evaluate the expression of RelA. Western blotting was used to analyze the level of NF-κB p65 protein, RESULTS ： ① By using immunohistochemical analysis, RelA was mainly localized in cytosol in normal cervical tissue and low - grade squamous intraepithelial lesions, whereas in high -grade lesions and squamous cell carcinomas, RelA translocated into the nucleus. ② By Western blotting analysis, RelA was detected in the cytosolic extracts in normal or LSILs. In cancer tissues, the expression of Re- 1A increased in nuclear extracts while their expression in the cytosolic extracts was relatively less. CONCLUSIONS ： Constitutive activation of NF-κB p65 may lead to oncogenesis. NF-κB p65 may be a new target for the treatment of human cervical squmous cancer.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第9期1775-1778,共4页
Chinese Journal of Pathophysiology
