摘要
本文用蛋白质免疫印迹方法发现了猴肝细胞膜上脂蛋白（ａ）受体，为进一步研究这一导致动脉粥样硬化的脂蛋白的致病机理提供了新的研究内容，具体方法为：取新鲜猴肝匀浆、离心、超声波等处理制得可溶性的膜受体粗提液，经十二烷基磺酸钠－聚丙烯酰胺凝胶电泳纯化后转移至硝酸纤维膜，用蛋白质免疫印迹方法检测，出现林异的脂蛋白（ａ）受体条带（统计学处理，～３００ｋＤａ）。
Lipoprotein(a)receptors on monkeyliver cell ular membranes were round by Western blotting.Since lipoprotein (a) is structural homology to plasminogen, the lipoprotein(a)receptors were further verified by plasminogen antibOdies.The experiments were conducted as follows :first ,the primary extract of the membrane receptors were made from fresh monkey liver by homogenaion, centrifugation and ultrasonic treatment etc.Soon,the extract was separated by electrophoresis and transfered Onto nitrocellulose(NC) filters.Then,lipoprotein(a)purified from human plasma bound with high affinity to isolated China rhesus monkey liver cellular membrane lipoprotein receptors on nitrocellulose blots and in a solid-phase assay, and it was further verified by plasminogen antibOdies. The results showed that there existed lipoprotein(a) receptors.Thus we conclude that lipoprotein (a) has its own catabolism pathway.
出处
《中国动脉硬化杂志》
CAS
CSCD
1996年第4期288-290,共3页
Chinese Journal of Arteriosclerosis
关键词
脂蛋白(a)受体
动脉粥样硬化
lipoprotein (a) receptor
ligand
lipoprotein( a )-specific antibody
plasminogen
Western blotting
