摘要
采用改良后的CTAB法，对山葡萄、软枣猕猴桃、蒙古栎、核桃楸、西伯利亚红松和偃松基因组DNA进行提取。结果表明，所提基因组DNA分子量与XDNA（48kb）接近，其紫外吸收比在1．66～1．89之间。第3次和第4次上清提取的DNA质量优于第1次和第2次。从提取产量看，每克鲜重提取DNA量最小为15μg·g^-1（核桃楸第4次上清），最高的为272μg·g^-1（山葡萄第3次上清）。西伯利亚红松和偃松第1次和第2次上清基本未提出DNA，第3次和第4次上清中得到了较高质量的DNA。经酶切鉴定和PCR扩增，所提的基因组DNA可以用于进一步研究。
Genomic DNA was extracted from leaves of Vitis amurensis, Actinidia arguta, Quercus mongolica, Juglans mandshurica and needles of Pinus sibirica, Pinus pumila by the method of modified CTAB. The isolated genomic DNA length was similar to λDNA （48 kb） and is suitable for both PCR amplification and digestion with restriction endonucleases. The absorbance ration （A260/A280 ） ranged 1.66 - 1.89. The quality of DNA extracted from third and fourth times suspension was better than from first and second times suspension. The yields of DNA ranged from 15μg· g^-1 fresh mass（from fourth time suspension J. mandshuric） to 272μg · g^-1 fresh mass （from third time suspension of V. amurensis ）. Although from first and second times suspension of P. pumila and P. sibirica wasn＇ t isolated genomic DNA, but from third and fourth times suspension was obtained high quality genomic DNA. The genomic DNA can be used for next analysis via identification of PCR and digestion with restriction endonucleases.
出处
《植物研究》
CAS
CSCD
北大核心
2006年第5期589-594,共6页
Bulletin of Botanical Research
基金
黑龙江省博士后科研基金项目
东北林业大学科研启动基金项目资助
