基质金属蛋白酶在新生兔机械通气炎性反应中的作用被引量：4

Role of Matrix Metalloproteinase in Ventilator-Induced Lung Injury Inflammatory Reaction of Newborn Rabbits

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摘要目的研究基质金属蛋白酶(MMPs)在新生兔机械通气肺损伤中的作用。方法新生兔75只,分为正常对照组3只,余下72只按2×2×3析因设计随机分为在高体积分数氧(100%)、低体积分数氧(45%)组,每组又分为高吸气峰压(PIP)、低PIP组共4组进行机械通气。通气中选取1、3、6 h 3个时间点活杀取肺,ELISA法检测肺组织匀浆MMP-2、MMP-9水平,同时测肺湿、干比,并结合病理片分析。结果1.高氧组MMP-2水平增加,同正常组相比有显著性差异。2.高氧组MMP-9均值低于正常组,有显著性差异;低氧组MMP-9均值高于正常组,但差异无意义;不同的PIP对MMP-9的影响无意义。3.MMP-2与MMP-9间呈正相关,同肺组织湿、干比呈正相关。结论新生兔机械通气6 h内,高氧通气能上调MMP-2,下调MMP-9,通气时机械拉伸上调MMP-2。机械通气影响MMPs合成,MMPs在新生兔机械通气肺损伤中发挥重要作用。 Objective To investigate the effect of matrix metalloproteinases（MMPs） in ventilator- induced lung injury inflammatory reaction of newhom rabbits. Methods Seventy - five newhorn rabbits aged 1 - 5 days were randomly arranged to 3 groups：control group （ n = 3） ,other 72 rabbits designed by 2 × 2 × 3 factorial were divided into high concentration oxygen （ 100% ）and low concentration oxygen（45% ） groups,and each group was subdivided into 2 groups： High peak inspiratory pressure （PIP）group and low PIP group. All rabbits were done by mechanical ventilation and killed to obtain lung tissue at 1,3,6 hours after trailing respectively. The concentration of MMP - 9 and MMP- 2 in lung tissue homogenate were detected by ELISA, at the same time the ratio of wet to dry was detected and pathological section was analyzed, Results 1. The concentration of MMP - 2 in high concentration oxygen group, high PIP group and low PIP group increased compared with that in normal group, and there were significant differences. 2, The mean concentration of MMP - 9 was lower in high concentration oxygen group than that in normal control group, and there was significant difference between 2 groups. The mean concentration of MMP - 9 in low concentration oxygen group was higher than that in normal group, and there was no significant difference between 2 groups. There was no significant difference in effect to mean concentration of MMP - 9 by different PIP. 3. There were positive correlation between MMP - 2 and MMP - 9 , MMP - 2 and the ratio of wet to dry in lung. Conclusions Within 6 hours in newhorn rabbits by mechanical ventilation, ventilation with high concentration oxygen up - regulation MMP - 2, but downregulated MMP - 9. The lungs stretch by mechanical ventilation increase MMP - 9, but decrease MMP - 2. Mechanical ventilation can affect the synthesis of MMPs,and MMPs plays an important role in ventilator- induced lung injury of newhorn rabbits.

作者花少栋杜江安胜利杨丽华封志纯

机构地区南方医科大学珠江医院儿科南方医科大学基础部统计教研室

出处《实用儿科临床杂志》 CAS CSCD 北大核心 2006年第18期1239-1241,共3页 Journal of Applied Clinical Pediatrics

基金广东省科技计划项目资助(B30502)

关键词基质金属蛋白酶兔新生通气机机械肺损伤 matrix metalloproteinase rabbits,newbom ventilation,mechanical lung injury

分类号 R722.1 [医药卫生—儿科]

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