摘要
目的观察一氧化氮合酶(NOS)抑制剂N-硝基精氨酸甲酯(L-NAME)抑制大肠癌生长的体内效应,及其对大肠癌移植瘤相关血管形成的影响。方法将20只BALB/c(nu/nu)裸鼠按随机数字表法均分为对照组和实验组,大肠癌细胞系SL174T种植入裸鼠皮下,形成移植瘤。对照组0.2ml生理盐水灌胃,实验组经口灌入L-NAME,4mg/次,3次/周,共4周。然后记录肿瘤大小,采用免疫组化法检测微血管密度。结果L-NAME对裸鼠大肠癌移植瘤的肿瘤重量抑瘤率为41.36%;肿瘤体积抑瘤率为43.48%。实验组大肠癌组织微血管密度(14.83±2.10)个,明显低于对照组的(21.04±3.11)个,两组间差异有统计学意义(P<0.05)。结论L-NAME具有抑制裸鼠大肠癌肿瘤生长和对抗肿瘤新生血管生成的作用。
Objective To investigate the effect of N^G- nitro-L-arginine methyl ester （L-NAME）, an inhibitor of nitric oxide synthase, on the growth of colorectal cancer xenografts in vivo and on tumor-associated neovascularization. Methods Twenty BALB/c nude mice were randomly divided into control group and study group equally. Human colorectal cancer cell line SL174T was inoculated subcutaneously into nude mice to form transplantation tumors. Saline 0.2 ml was intragastric-administrated to mice in control group and L-NAME （4 mg toties guoties） was administrated orally to mice in study group three times per week for four weeks. The changes of tumors in both groups were recorded and the microvessel density （MVD） was also measured by immunohistochemistry assay. Results L-NAME significantly inhibited the growth of colorectal cancer xenografts in nude mice. The weight of transplantation tumor reduced with the inhibitory rate of 41. 36%, and the inhibitory rate of tumor volume was 43.48% in study group. MVI） in the study group and control group were 14.83±2. 10 and 21.04±3. 11, respectivety, which showed that the former was significantly lower than that of the control group （P〈0.05）. Conclusion L-NAME may inhibit the growth of colorectal cancer via the suppression of tumor neovascularization.
出处
《中国普外基础与临床杂志》
CAS
2006年第5期542-544,共3页
Chinese Journal of Bases and Clinics In General Surgery
基金
黑龙江省青年科学技术专项资金项目(编号:QC05C39)
