摘要
利用花粉管通道技术将甜菜碱醛脱氢酶(BADH)基因导入玉米,以提高玉米的耐盐性.对1 286株转化玉米进行PCR检测,共有16株呈阳性,转化率为1.2%.Southern Blot进一步证明BADH基因已经整合到玉米基因组.将阳性植株的后代(T3)用含1.2%NaCl的Hogland营养液每日1次浇灌,14 d后对玉米叶片的相对电导率和叶绿素含量进行测定.结果表明在同样的盐胁迫条件下,转基因植株所受到的盐伤害明显较对照植株轻,说明转入的BADH基因可以提高玉米的耐盐能力.
Betaine aldehyde dehydrogenase （BADH） gene was transferred into Maize by using pollentube pathway method in order to enhance the salt tolerance of Maize. Investigation by PCR, shows that 16 of 1 286 transformed maizes are positive and the efficiency of transformation is 1.2%. Southern Blot indicated that BADH gene had been integrated into the genome of Maize. T3 of positive Maize was planted in Hogland culture medium with 1.2 % NaCl. The relative conductivity and chlorophyll content of the Maize was analyzed after growing for 14 days. The result of the experiment proved that the damage of salt to transgenic Maize was lower than the control under the same concentration treatment of salt, and it showed that the transformed BADH gene enhanced the salt tolerance of Maize.
出处
《辽宁师范大学学报（自然科学版）》
CAS
北大核心
2006年第3期344-347,共4页
Journal of Liaoning Normal University:Natural Science Edition
基金
辽宁省科技基金资助项目(2001101001)
关键词
花粉管通道
耐盐性
玉米
遗传转化
BADH
pollen-tube pathway
salt-tolerance
Maize genetic transformation
BADH
