摘要
文章对绞股蓝主要化学成分黄酮类化合物和总皂甙进行了分析测定。这两种化合物在香草醛-高氯酸作用显色后加入冰醋酸，分别在451和547nm处有良好的吸收，但吸收曲线相互重叠，难以同时测定。采用系数倍率-光谱法以芦丁和人参皂甙Rbl为对照品，对绞股蓝中的总黄酮和总皂甙进行分析，以达到同时测定的目的。所得回归方程分别为：△A黄=0．0133＋4．4170c黄，相关系数r黄=0．9994，黄酮的浓度0～0．16μg·mL^-1范围内，△A黄与浓度c黄之间呈良好的线性关系；△A皂=2．7755c皂-0．8881×10^-2，相关系数r皂=0．9991，在总皂甙的浓度0～0．30μg·mL^-1范围内，△A皂与浓度c皂之间呈良好的线性关系。它们可按标准曲线法进行定量分析。该法的回收率分别为104．0％～113．0％，86．8％～94．6．0％，相对标准偏差分别小于0．58％（n=9），0．35％（n=9）。方法简便，快速，准确而且操作简便易行。
Important chemical constituents analysis for the total flavone and total saponin in gynostemma pentaphyllum is described. The colour reactions of flavones and saponines with vanillin-perchloric acid in acetic acid produced the good absorptions at 451 and 547 nm, but the absorption peaks too overlapped to be determined simultaneously. A new method for the total flavone and the total saponin in gynostemma pentaphyllum to be determined by signal multiplier spectrophotometry simultaneously without any preliminary separation was proposed. For quantitative analysis, the rutinum as a standard of the total flavone and the ginsenoside Rbl as standard of the total saponin were applied. The experiment results showed that the regression equations of concentration and △A were obtained： △Aflavone =0. 013 3＋4. 417 0cflavone,relation coefficient rflavone=0. 999 4, and the total flavone concentrations were in 0-0. 16μg·mL^-1 with △A obeying linear relation; △Asaporin =2. 775 5csaporin-0. 888 1 × 10^-1, relation coefficient rsaporin =0. 999 1, and the total saponin concentrations were in 0-0.30μg·mL^-1 with △A obeying linear relation respectively. The recovery ratio was 104. 0%-113.0 % and 86.8%-94.6% respectively. The RSDflavone was less than 0.58%（n= 9） and RSDsaporin was less than 0.35%（n=9） respectively. The proposed method is simple, rapid accurate and feasible.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2006年第9期1753-1756,共4页
Spectroscopy and Spectral Analysis
基金
辽宁省教育厅科学技术基金课题(2024201050)资助
关键词
系数倍率-光谱法
绞股蓝
总皂甙
总黄酮
定量测定
Signal multiplier spectrophotometry
Gynostemma pentaphyllum
Total saponin
Total flavone
Determination
