摘要
利用重组戊型肝炎病毒（HEV）衣壳蛋白片段p239（368～606aa）形成的类病毒颗粒作为亲和层析诱饵蛋白，HepG2为细胞模型，筛选与p239类病毒颗粒特异性相互作用蛋白：经过二维电泳分离，MALDI-TOF-MS分析鉴定得到GRP78／Bip，HSP90，alpha-tubulin及P43四个与p239有相互作用的候选蛋白。GRP78／Bip为热休克蛋白家族成员，体外免疫共沉淀实验结果证实，其与p239有特异性的结合：此研究结果为深入研究HEV的感染过程如吸附、入胞，以及HEV的致病机理提供了有益线索。
For further understanding the infection mechanism and pathogenesis of hepatitis E virus （HEV）, we screened the protein interacting with HEV virus- like particle （p239） extracted from HepG2 cells by affinity chromatography, two-dimensional electrophoresis and mass spectrometry. Four candidate proteins, GRP78/ Bip, HSP90, alpha-tubulin and P43 were identified. The interaction between GRP78/Bip and p239 was further confirmed by co-immunoprecipitation. Our results suggest that GRP78/Bip may contribute to the cell attachment or particle assembly of HEV.
出处
《病毒学报》
CAS
CSCD
北大核心
2006年第5期329-333,共5页
Chinese Journal of Virology
基金
863计划(2005AA2Z3H20)
福建省科技重大专项(2004YZ01)
厦门市重大产业科技项目(3502Z20041008)
