期刊文献+

家兔急性鼻窦炎鼻源性模型的建立 被引量:7

The establishment of a rhinogenic model of acute sinusitis in rabbits
下载PDF
导出
摘要 目的探讨在家兔鼻腔填塞膨胀海绵并植入细菌以制备急性鼻窦炎鼻源性动物模型的方法。方法健康成年雄性新西兰白兔30只,随机抽取5只处死,测量其右侧鼻腔前鼻孔上下径、内外径以及前鼻孔至上颌窦自然开口的距离,余25只分成模型组(10只)、单纯堵塞组(10只)和空白对照组(5只)。将2.5 mm×5 mm×28 mm大小Merocel高分子膨胀海绵置入模型组、单纯堵塞组兔右侧鼻腔,同时向模型组兔鼻腔内高分子膨胀海绵中注射植入1 mlⅢ型肺炎链球菌悬液,单纯堵塞组注射等量生理盐水。第10天行鼻窦CT扫描后将兔处死,分别取右上颌窦、筛窦窦腔分泌物行细菌培养,并将右上颌窦、筛窦完整取出,全组织包埋行组织病理学检查。结果除模型组1只于第4天死亡外,其余均存活。模型组家兔CT检查均示右上颌窦、筛窦腔不同程度浑浊化,鼻窦腔内分泌物细菌培养阳性率为100%,光镜观察可见窦腔大量中性粒细胞聚积,上皮变性,局部溃疡形成,黏膜内及黏膜下大量炎性细胞浸润。单纯堵塞组8只家兔可见类似的影像学改变,但病理学改变程度较轻,与模型组相比,差别有统计学意义。结论成功建立了家兔急性鼻窦炎的鼻源性动物模型,为进一步研究鼻源性鼻窦炎的发病机制及治疗干预奠定基础。 Objective To create a rhinogenic model of acute sinusitis using an implanted sponge impregnated with pathogenic bacteria in the nasal cavity of the rabbits. Methods Thirty healthy adult male New Zealand white rabbits were used. Five of them were chosen randomly for the measurement of the distances between superior to inferior edge and exterior to interior edge of anterior naris of the right-side nasal cavity and the distance between anterior naris and maxillary ostium. The other 25 were divided into modeling group (10 rabbits), simple blocking group (10 rabbits) and blank control group (5 rabbits). A piece of polyvinyl acetal absorbent sponge (Merocel) in size of 2.5 mm × 5 mm × 28 mm was inserted into the right-side nasal cavity of the rabbits in both modeling and simple blocking groups. The sponges were soaked with 1 ml type Ⅲ streptococcus pneumoniae solution and 1ml sterile saline solution in modeling group and simple blocking group respectively. At the 10th day after insertion, the sinuses were examined by computed tomography scans and then all rabbits were sacrificed to obtain the secretions from the right-side maxillary and ethmoidal sinuses for culture, followed by the block remove of the right sinuses for whole-mount histological analyses. Results At the 10th day after insertion, all rabbits survived except one of modeling group died at the 4th day. In modeling group, the positive rate of bacterial culture were 100 % in secretions from both the maxillary and ethrnoidal sinuses. CT scan showed opacification of right maxillary sinuses and ethmoidal sinuses. Lumenal predominant neutrophilic leukocytes accumulation was observed under light microscope. Epithelial degeneration with ulceration in some areas, and inflammatory ceils infiltration of the mucous layer as well as the submucosa were also observed. Eight rabbits in simple blocking group had similar radiological changes, but histopathological changes were lighter in degree than those in modeling group. The results were significantly different between the two groups. Conclusion The rhinogenic model of acute sinusitis has been successfully established in the rabbits, which lays a foundation for the further researches on the pathogenesis and therapia of rhinogenic sinusitis.
出处 《中国耳鼻咽喉颅底外科杂志》 CAS 2006年第4期248-253,共6页 Chinese Journal of Otorhinolaryngology-skull Base Surgery
基金 教育部留学回国人员科研基金资助项目(批准号:教外司留1998679)
关键词 鼻窦炎 疾病模型 动物 肺炎链球菌 Sinusitis Disease models, animal Streptococcus pneumoniae
  • 相关文献

参考文献8

  • 1Piccirillo JF.Clinical practice.Acute bacterial sinusitis[J].N Engl J Med,2004,351(9):902-910.
  • 2Kim CS,Jeon SY,Min YG,et al.Effects of beta-toxin of Staphylococcus aureus on ciliary activity of nasal epithelial cells[J].Laryngoscope,2000,110(12):2085-2088.
  • 3Kumlien J,Schiratzki H.Blood flow in the rabbit sinus mucosa during experimentally induced chronic sinusitis.Measurement with a diffusible and with a non-diffusible tracer[J].Acta Otolaryngol,1985,99(5-6):630-636.
  • 4Hinni ML,McCaffrey TV,Kasperbauer JL.Early mucosal changes in experimental sinusitis[J].Otolaryngol Head Neck Surg,1992,107(4):537-548.
  • 5Marks SC.Acute sinusitis in the rabbit:a new rhinogenic model[J].Laryngoscope,1997,107(12):1579-1585.
  • 6林彬,林心强,王挥戈.鼻窦炎的动物模型[J].中国中西医结合耳鼻咽喉科杂志,2004,12(1):46-48. 被引量:7
  • 7Kara CO,Cetin CB,Demirkan N,et al.Experimental sinusitis in a rhinogenic model[J].Laryngoscope,2004,114(2):273-278.
  • 8刘锋,许育,周水淼,张速勤,郑宏良.急慢性鼻窦炎中肺炎链球菌检验方法的改进[J].临床耳鼻咽喉科杂志,2002,16(2):84-85. 被引量:2

二级参考文献26

  • 1[13]Fukami M,Norlander T,Stierna P, et al. Mucosal pathology in the nose and sinuses :a study in experimental maxillary sinusitis in rabbits induced by Streptococcus pneumoniae, Bacteroides fragilis, and Staphylococcus aureus. Am J Rhinol, 1993,7:125-132.
  • 2[14]Westrin KM,Stierna P,Carlson B,et al. Mucosal fine structure in experimental sinusitis. Ann Otol Rhinol Laryngol, 1993,102: 639-645.
  • 3[15]Norlander T,Fukami M,Westrin KM,et al. Formation of mucosal polyps in the nasal and Maxillary sinus cavities by infection. Otolaryngol Head Neck Surg,1993,109(3) :522-529.
  • 4[16]Min YG,Lee YM ,Lee BJ,et al. The effect of ostial opening on experimental maxillary sinusitis in rabbits.Rhinology, 1993,31:101-105.
  • 5[17]Benninger MS,Kaczor J,Stone C. Natural ostiotomy vs. inferior antrostomy in the management of sinusitis:an animal model. Otolaryngol Head Neck Surg, 1993,109(6): 1035-1042.
  • 6[18]Bende M,Arfors KE,Stierna P,et al. Effect of oxymetazoline nose drops on acute sinusitis in the rabbit. Ann Otol Rhinol Laryngol, 1996,105: 222-225.
  • 7[19]Karin Forsgren. Effects of surgery on mucosal pathologic changes following experimental sinusitis in rabbit.Ann Otol Rhinol Laryngol, 1998,107:155-163.
  • 8[20]Steven C,Marks MD. Acute sinusitis in the rabbit Model: Histologic Analysis. Laryngoscope. , 1998, 3: 320-325.
  • 9[21]Abraham Jacob MD,Brian T Faddis, PhD Richard A,et al. Chronic bacterial rhinosinusitis:description of a mouse model. Arch Otolaryngol Head Neck Surg, 2001,127:657-663.
  • 10[22]Bomer K,Brichta A,k Baroody F,et al. A mouse model of acute bacterial rhinosinusitis. Arch Otolaryngol Head Neck Surg, 1998,124:1227-1232.

共引文献7

同被引文献46

  • 1萧建新,李润汉,招焕婵,刘悦胜,唐隽.鼻窦内镜术后填塞不同材料的效果比较[J].医学理论与实践,2006,19(8):896-898. 被引量:6
  • 2陈秀梅,张庆泉,栾信庸.三种鼻腔填塞材料在鼻内镜手术中的疗效比较[J].山东大学耳鼻喉眼学报,2006,20(5):438-440. 被引量:13
  • 3卫红齐,李泽卿,王秋萍,周玫,程向荣,王天友.家兔急性鼻窦炎模型两种建模方法的比较[J].中国比较医学杂志,2007,17(4):201-205. 被引量:3
  • 4卫红齐,王秋萍,李泽卿,周玫,陈伟,吴昆旻.家兔急性鼻窦炎上颌窦黏膜病理改变[J].中国耳鼻咽喉头颈外科,2007,14(7):431-434. 被引量:4
  • 5Bhattacharyya N. Bacterial infection in chronic rhinosinusitis : a controlled paired analysis [ J ] . Am J Rhinol, 2005 , 19 (6) : 544 -548.
  • 6Park SS, Samiy N, Ruoff K, et al. Effect of intravitreal dexamethasone in treatment of pneumececcal endophthalmitis in rabbits [ J ]. Arch Ophthalmol, 1995, 113 (10) : 1324 - 1329.
  • 7Dolor RJ, Witsell DL, Hellkamp AS, et al. Comparison of cefuroxime with or without intranasal fluticasone for the treatment of rhinosinusitis. The GAFFS Trial : a randomized controlled trial[J]. JAMA, 2001, 286 (24) : 3097 - 3105.
  • 8Nayak AS, Settipane GA, Pedinoff A, et al. Effective dose range of mometasone furoate nasal spray in the treatment of acute rhinosinusltis [ J ]. Ann Allergy Asthma Immunol, 2002, 89(3): 271 -278.
  • 9Min YG, Kim YK, Choi YS, et al. Mucociliary activity and histopathology of sinus mucosa in experimental maxillary sinusitis : a comparison of systemic administration of antibiotic and antibiotic delivery by polylactic acid polymer [ J ] . Laryngoscope, 1995, 105(8 ): 835-842.
  • 10Kim CS, Jeon SY, Min YG, et al. Effects of beta - toxin of staphylococcus aureus on ciliary activity of nasal epithelial cells[ J ]. Laryngoscope, 2000, 110 (12) : 2085 - 2088.

引证文献7

二级引证文献20

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部