摘要
目的检测原发性肾病综合征(PNS)患儿尿液结缔组织生长因子(CTGF)水平,评价其临床意义。方法采用双抗体夹心ELISA方法检测42例接受治疗的PNS患儿治疗前及缓解后尿液CTGF水平,并与28例健康对照组比较。结果激素敏感型24例患儿治疗后尿液CTGF水平明显下降(t=34.26 P<0.01)。激素抵抗型18例患儿治疗后尿液CTGF水平无明显变化(t=1.17 P>0.05);加用环磷酰胺冲击治疗后12例病情缓解,尿CTGF水平明显下调(t=3.68 P<0.01)。CTGF水平与尿清蛋白(r=0.51 P<0.01)、24 h尿蛋白(r=0.43 P<0.01)呈正相关。结论尿液CTGF水平可作为反映NS病情、评价治疗效应的指标。
Objective To measure the levels of urinary/xmnective tissue growth factor（CTGF） and evaluate its clinical significances in children with primary nephritic syndrome（PNS）. Methods The urinary CTGF levels were measured in 42 children with PNS, which were treated with prednisone or/and cyclophosphamide by double - antibody ,sandwich ,sensitive enzyme linked immunosorbent assay （EIASA） and compared with those of healthy controls（ n - 28）. Results Twenty four patients responded ,sensitively to prednisone, whose urinary CTGF was decreased after complete remission by prednisone treatment [（73. 31 ± 15.23 ） vs （54. 82 ± 14. 84 ） ng/（mg·Cr） P〈0.01 ]. In 18 patients with steroid resistant, the levels of urinary CTGF did not decreased after standard course of treatment with prednisome[（74.71 ± 16.46） vs （68.79±13.78） ng/（mg·Cr） P〉0.05],however,complete remission of PNS as cyclophosphamide pulse treatment was added in 12 patterns[ （72.64 ± 13.80） vs （52.17±12.46） ng/（mg·Cr） P〈0.01 ）]. The levels of urinary （TGF showed a positive correlation with albumin（ r = 0.51 P〈0.01 ） ,and 24 h-total urine protein excretion（ r = 0.43 P〈0.01 ）. Conclusion The levels of urinary CTGF may be considered as a predictor on reflecting disease activity and estimating thera-peutic effect in patients with PNS.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2006年第17期1143-1144,共2页
Journal of Applied Clinical Pediatrics
关键词
结缔组织
生长物质
肾病综合征
儿童
connective tissue, growth substances
nephritic syndrome
child
