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中药黄芩苷对脂多糖刺激人牙周膜成纤维细胞分泌肿瘤坏死因子-α的影响 被引量:12

Effects of bacailin on TNF-αproduction of human periodontal ligament fibroblasts by stimulation of LPS
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摘要 目的研究中药黄芩苷对脂多糖(LPS)刺激人牙周膜成纤维细胞(HPLFs)分泌肿瘤坏死因子-α(TNF-α)的影响。方法采用原代培养HPLFs技术和酶联免疫检测方法(ELISA),检测培养上清液中TNF-α的水平。结果0.001~10μg/ml黄芩苷和2μg/ml地塞米松均可显著抑制LPS刺激HPLFs分泌TNF-α的活性(P<0.01),而不同浓度组之间比较,抑制作用无显著性差异(P>0.05)。结论黄芩苷对LPS刺激HPLFs合成和分泌TNF-α有显著抑制作用,与地塞米松抗炎效果相近,作用机制与抑制TNF-α等炎性细胞因子过度产生有关,揭示了黄芩苷可能的抗炎作用机制。 Objective To study the effects of the Chinese baicalin on human periodontal ligament fibroblasts (HPLFs) secreting tumor necrosis factor alpha (TNF-α) stimulated by lipopolysaccharide (LPS) of Eseherichia coil (E. coil). Methods The cell culture technique in vitro, Enzyme- linked immunosorbent assay (ELISA) were used to observe the effects of baicalin on the level of TNF -α in culture supernatant. Results Baicalin and dexamethasone could significantly inhibit the activity of TNF-α induced by LPS and significantly suppressed TNF -α production of HPLFs stimulated with LPS ( P 〈 0.01 ), but among the different baicalin concentration groups and dexamethasone group, there is no significant difference ( P 〉 0.05 ). Conclusion Human periodontal ligament fibroblasts have the ability of synthesizing and secreting TNF -α , and LPS is a potent stimulation for HPLFs to generate TNF -α ; Baicalin and dexamethasone can significantly inhibit the TNF -α production of HPLFs stimulated by LPS and there is not significantly difference between both.
出处 《现代口腔医学杂志》 CAS CSCD 北大核心 2006年第5期531-535,共5页 Journal of Modern Stomatology
关键词 LPS 黄芩苷 人牙周膜成纤维细胞TNF-α Baicalin Lipopolysaccharide Human periodontal ligament fibroblasts Tumor necrosis factor alpha
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