摘要
目的应变率成像观察犬急性缺血再灌注后左心室局部功能,缺血局部心肌细胞凋亡及Caspase3表达的变化。方法健康杂种犬30只,分为对照组(10只),缺血组(10只),再灌注组(10只)。缺血组于第1对角支1cm以下套扎左冠状动脉前降支30min,再灌注组套扎30min后再灌注120min,对照组游离左冠状动脉前降支不结扎。超声心动图左室收缩期应变率测量左室局部收缩功能,TUNEL法检测缺血区心肌细胞凋亡指数,免疫组化法测缺血心肌细胞Caspase3的表达。结果结扎30min后,缺血组左室前壁缺血节段(中间段)收缩期应变率明显降低(-0.54±0.23)(P<0.01)。并出现收缩后压缩(postsystoliccompression,PSC),再灌注120min后缺血节段的应变率有所恢复(-0.89±0.43),但仍低于对照组(P<0.05)。缺血组缺血区心肌TUNEL阳性细胞指数与对照组比较无显著性差异(2.9±1.3比对照组2.7±1.7)(P>0.05);再灌注组缺血区心肌TUNEL阳性指数明显增加(25.1±3.3)(P<0.01)。缺血区心肌细胞Caspase3表达升高,(20.0±3.1比对照组4.7±2.4)(P<0.01),再灌注组Caspase3表达进一步增强(P<0.01)。结论急性心肌缺血再灌注促凋亡基因Capase3激活,缺血心肌细胞凋亡可能为其早期改变,应变率成像可以评价早期心肌局部收缩功能。
Objective To investigate left ventricular segmental function and alteration of myocardial apoptosis and caspase 3 following acute ischemia reperfusion in dogs. Methods Thirty dogs were divided into ischemia （n=10）, reperfusion （n=10）, and sham operation as control （n=10） groups. Left anterior descending coronary artery （LAD） were ligated for 30 rain while ligation was followed by reperfusion for 120 min in reperfusion group. The left ventricular segmental function were detected by echocardiography using strain rate analysis software. The apoptotic index （AI） in ischemia myocardium was detected by TUNEL, Caspase 3 by immunohistochemistry analysis. Results The peak strain rate （SRpeak） in middle segment of left ventricle in ischemia group was decreased obviously （-0. 54±0.23）（P〈0. 01）, with postsystolic compression （PSC）. SRpeak increased slightly after reperfusion 120 min（-0. 89±0.43）, but still lower than control group. No difference in positive rate of TUNEL in ischemia myocardium between ischemia group and control groups（2.9±1.3 vs control 2. 7±1.7 ） （P〉0.05）, but it was increased significantly in reperfusion groups （ 25. 1±3.3 ） （ P〈0.01）. Caspase 3 expression was increased in ischemia group and reperfusion group than control group（ischemia 20. 0±3. 1 vs control 4. 7±2.4） （P〈0.01）. Conclusion Caspase 3 expression and cell apoptosis maybe the early changes following acute ischemia reperfusion in dogs, which are related to the left ventricular segmental performance.
出处
《中华高血压杂志》
CAS
CSCD
北大核心
2006年第9期739-742,共4页
Chinese Journal of Hypertension
关键词
凋亡
心肌缺血再灌注
CASPASE
3
左心功能
Apoptosis
Myocardial ischemia reperfusion
Caspase 3
Left ventricular function
