促红细胞生成素对新生鼠缺血缺氧性脑损伤保护作用的研究被引量：8

Study on the protective role of erythropoietin in hypoxic-ischemic brain damage to neonatal rats.

下载PDF

导出

摘要【目的】探讨促红细胞生成素对缺血缺氧新生鼠脑损伤(hypoxic-ischemic brain damage,HIBD)的保护作用。【方法】7日龄Wistar大鼠102只,随机分成3组,①假手术组(n=18);②观察组(n=42):HIBD模型后即刻给促红细胞生成素3 000 U/kg腹腔注射;③对照组(n=42):HIBD模型后即刻腹腔注射等体积的生理盐水。于处置后2、6、12、24、48 h检测脑组织中超氧化物歧化酶(SOD)、丙二醛(MDA)水平;并于处置后48 h作HE和TUNEL染色光镜下检测脑细胞凋亡数,以及测定脑组织水含量。【结果】观察组脑组织SOD升高、MDA降低、脑细胞凋亡数及脑组织水含量减少,与对照组比较差异均有显著性(P<0.05)。【结论】促红细胞生成素可通过拮抗氧自由基、减轻脑水肿和阻止神经细胞凋亡,发挥对新生鼠HIBD时脑的保护作用。 [Objective] To explore the protective role of erythropoietin （EPO）in hypoxic-ischemic brain damage in neonatal rats. [Methods] 102 7-day-old Wistar rats were divided into the three groups：Sham operation,HIBD＋Normal Saline （NS）（control） and HIBD＋EPO （observation）. SOD activity, MDA level were measured in 2,6,12,24,48 h after the treatment of EPO or NS,and the brain water content and the number of the apoptic cells were observed. [Results] SOD decreased and MDA increased in HIBD＋ NS, but SOD increased and MDA decreased in HIBD＋ EPO. The number of cell apoptosis in HIBD＋EPO was much fewer than that of HIBD＋ NS. The water content of observation group was lower than that of the control group. [Conclusion] EPO might be useful to protect brain of neonatal rat against hypoxic-ischemic damage by resisting free redical, reducing brain edema and inhibiting cell apoptosisl.

作者陈广斌张毅陆羡

机构地区广东医学院附属医院儿科广东医学院附属医院中心实验室

出处《中国儿童保健杂志》 CAS 2006年第4期370-372,共3页 Chinese Journal of Child Health Care

关键词脑缺氧脑缺血促红细胞生成素自由基丙二醛细胞凋亡 cerebral anoxia cerebral ischemia erythropoietin SOD MDA apoptosis

分类号 R722.1 [医药卫生—儿科]

引文网络
相关文献

参考文献11

1RICE JE,VANNUCCI RC,BRIERLEY JB.The influence of immaturity on hypoxic-jschemic brain damage in the rat[J].Ann Neurol,1981,9:131-141.
2EDWARDS AD,YUE X,COX P,et al.Apoptosis in the brains of infants suffering intrauterine cerebral injury[J].Pediatr RES,1997,42:684-689.
3RENOLLEAU S,AGGOUN ZD,BEN-ARE Y,et al.A model of transient unilateral focal ischemia with reperfusion in the P7 neonatal rat morphological change indicative of apoptosis[J].Stroke,1998,29:1454-1461.
4JUUL S.Erythropoietin in the central nervous system,and its use to prevent hypoxic-ischemic brain damage[J].Acta Paediatr,2002,438:36-42.
5CHONG ZZ,LIN SH,KANG JQ,et al.Erythropoietin prevent early and late neuronal demise through modulation of akt1 and induction of caspase 1,3 and 8[J].J Neurosci Res,2003,71:659-669.
6CALAPAI G,MARCIANO MC,CORICA F,et al.Erythropoietin protects against brain ischemic injury by inhibition of nitric oxide formation[J].Eur J Pharmacol,2000,401:349-356.
7SAKANAKA M,WEN TC,MATSUDA S,et al.In vivo evidence that erythro-poietin protects neurons from ischemic damage[J].Proc Natl Acad Sci USA,1998,95:4635-4640.
8RUSCHER K,FREYER D,KARSCH M,et al.Erythropoietin is a paracrine mediator of ischemic tolerance in the brain:evidence from an in vivo model[J].J Neurosci,2002,22:10921-10923.
9BERNAUDIN M,MARTI HH,POUSSEL S,et al.Apotential role for erythro-poietin in focal permanent cerebral ischemia in mice[J].J Cereb Blood Flow Metab,1999,19:643-651.
10KUMRAL A,OZER E,YILMAZ O,et al.Neurop rotective effect of ery-thropoietin on hypoxic-ischemic brain injury in neonatal rats[J].Bio INeonate,2003,83:224-228.

二级参考文献10

1Brines ML, Ghezzi P, Keenan S, et al. Erythropoietin crosses the blood- brain barrier to protect against experimental brain injury[J ].Proc Natl Acad Sci USA, 2000,97( 19):10526- 10531.
2Neumann D, Yuk MH, Lodish HF, et al. Blocking intracellular intracellular degradation of the erythropoietin and asialoglycoprotein receptors by calpain inhibitors does not result in the memberane and secreted forms [J ]. Biochem J, 1996,313: 391 - 399.
3Grasso G, Buemi M, Alafaci C, et al. Beneficial effects of systemic administration of recombinant human erythropoetin in rabbits subjected to subarachnoid hemorrhage[J ]. Proc Natl Acad Sci USA,2002,99: 5627 - 5631.
4Martinez- Estrada OM, Rodriguez- Millan E, de Vicente EG, et al. Erythropoietin protects the in vitro blood- brain barrier against VEGF- induced permeability [J ]. Eur J Neuroc, 2003,18: 2538 -2544.
5Juul SE, McPherson RJ, Farrell FX, et al. Erythropoietin concentrations in cerebrospinal fluid of nonhuman primates and fetal sheep following high - dose recombinant erythropoietin [J ]. Biol Neonate,2004,85:138 - 144.
6Springborg JB, Ma X, Rochat P, et al. A single subcutaneous bolus of erythropoietin normalizes cerebral blood flow autoregulation after subarachnoid haemorrhage in rats [J ]. Br J Pharmacol,2002,135:823 - 829.
7Kumral A, Ozer E, Yilmaz O, et al. Neuroprotective effect of erythropoietin on hypoxic - ischemic brain injury in neonatalrats [J ].Biol Neonate, 2003,83: 224 - 228.
8Eid T, Brines M. Recombinant human erythropoetin for neuroprotection: What is the evidence[J ]. Clin Breast Cancer Suppl, 2002,3(3):109- 115.
9唐瑟,姚裕家,陈永秀,陈娟.重组人促红细胞生成素对早产儿贫血和细胞免疫功能的影响[J].实用儿科临床杂志,2002,17(4):292-293. 被引量：4
10张新华,吴志奎,黄有文,王荣新,蔡辉国,黄欣秋,蔡利群,李平萍,朱凌,周天红.珠蛋白生成障碍性贫血患儿血清促红细胞生成素变化[J].实用儿科临床杂志,2003,18(5):339-340. 被引量：3