摘要
成年雌性新西兰家兔１２只，术前测定血清的骨钙素含量为３６３．６７±８０．９５ｎｇ／ｍｌ，碱性磷酸酶、酸性磷酸酶和骨特异性碱性磷酸酶的活性分别为２０．０６±８．２０Ｕ／Ｌ、１９．０６±５．４６Ｕ／Ｌ、１４．０４±１．６７Ｕ／Ｌ。摘除二侧卵巢８月后，家兔血清的骨钙素含量为３２７．５０±８７．２９ｎｇ／ｍｌ，碱性磷酸酶、酸性磷酸酶和骨特异性碱性磷酸酶的活性分别为１４．６０±４．６４Ｕ／Ｌ、１１．０４±４．１０Ｕ／Ｌ、４．４４±２．１０Ｕ／Ｌ。摘除卵巢术后各指标均显著降低（Ｐ＜０．００１）。骨特异性碱性磷酸酶活性降低的幅度比碱性磷酸酶大，因此骨特异性碱性磷酸酶可作为特异和灵敏的骨代谢指标。
Twelve female New Zealand rabbits of seven months of age, each weighing about 3kg,were subjected to oophorectomy The rabbit serum was examined for osteocalcin, alkaline phosphatase, acid phosphatase and bone-specific alkaline phosphatase before and 8 monthe after oophorectomy. The serum osteocalcin content was 363. 67±80. 95ng/ml in intact animals and dropped to 327. 50±87. 29 ng/ml 8 months after oophorectomy, the difference being statistically significant(P<0.001). The alkaline phosphatase, acid phosphatase and bone-specific alkaline phosphatase activities of serum were 20.06±8.20U/L, 19.06±5.46U/L, and 14.04±1.67 U/L respectively in intact animals and declined to 14.60 ±4.60 U/L,11.04±4. 10U/L, and4. 44±2.10U/L respectively 8 months after oophorectomy. The alkaline phosphatase, acid phosphatase and bone-specific alkaline phosphatase activities in intact animals were significantly higher than those of the oophorectomized animals (P<0.001). Since the decline in bone-specific alkaline phosphatase activity far exceeds that of alkaline phosphatase activity, the former can be used as a sensitive specific marker of bone metabolism.
出处
《上海第二医科大学学报》
CSCD
1996年第6期427-429,共3页
Acta Universitatis Medicinalis Secondae Shanghai
基金
上海市科委基金
关键词
卵巢
骨钙素
碱性磷酸酶
酸性磷酸酶
骨质疏松
oophorectomy
osteocalcin
alkaline phosphatase
acid phosphatase
bone-specific alkaline phosphatase
rabbit
