摘要
建立了一种采用KYA H IQ sil C18柱(250 mm×4.6 mm,5μm),以乙腈-水溶液(体积比1∶9)为流动相,在流速1.0 mL/m in、检测波长280 nm、进样量10μL的条件下,用高效液相色谱同时测定红景天苷及其苷元酪醇含量的方法。利用均匀试验设计研究了分析样品的制备方法,确定了红景天根粉的最佳提取条件为:以蒸馏水为溶剂在60℃下超声波提取50 m in。分别测定了不同产地的红景天属植物中红景天苷和酪醇的含量,西藏产圣地红景天中红景天苷含量最高,达到2.147%,其次为藏产大花红景天和西藏红景天,分别为1.763%和1.271%;四川产大花红景天中酪醇含量最高,为0.298 5%,其次是藏产西藏红景天和大花红景天,分别为0.214 3%和0.183 6%;长鞭红景天和狭叶红景天中2种有效成分的含量均很低,红景天苷含量仅为0.034%和0.003%,酪醇含量则分别为0.016 4%和0.004 8%。
A method for the determination of contents of salidroside and tyrosol in Rhodiola by HPLC was established. The HPLC separation was preformed on a KYA HIQ sil C18 column （250 mm×4.6 mm,5 μm）, at flow rate 1 mL/min,with mobile phase of acetonitrile/water （ 1 ： 9） and detected at wavelength 280 nm. Using uniform design, the optimal conditions for salidroside and tyrosol extraction were explored to be ultrasonically extracted by distilled water at 60 ℃ for 50 min. Contents of salidroside and tyrosol in Rhodiola roots were determined. Salidroside content was 2. 147 % , the highest in Rh. sacra from Tibet, then in Rh. crenulata and Rh. tibetica from Tibet, 1. 763 % and 1. 271%, respectively; tyrosol content was 0.298 5 % ,the highest in Rh. crenulata from Sichuan, then in Rh. tibetica and Rh. crenulata from Tibet, 0. 214 3 % and 0. 183 6 %, respectively; whereas both contents were very low in Rh. fastigiata and Rh. kirilowii from Sichuan with salidroside contents of only 0.034 % and 0.003 %, and tyrosol contents of 0.016 4 % and 0.004 8 %, respectively.
出处
《林产化学与工业》
EI
CAS
CSCD
2006年第3期51-54,共4页
Chemistry and Industry of Forest Products
基金
国家自然科学基金资助项目(39700013)
关键词
红景天
红景天苷
酪醇
超声波提取
Rhodiola
salidroside
tyrosol
ultrasonic extraction
