摘要
目的探讨益气活血复方对自发性高血压大鼠(spontaneously hypertensive rats,SHR)左室肥厚(left ventricu lar hy-pertrophy,LVH)及左室心肌形态结构的影响。方法雄性14周龄SHR 50只,随机分为复方高剂量组(SHR-Y1)、复方中剂量组(SHR-Y2)、复方低剂量组(SHR-Y3)、模型对照组(SHR-C)、硝苯地平组(SHR-X)。监测尾动脉收缩压(systolicb lood pressure,SBP),每4周1次,8周后取材。测算心脏重量指数(heartwe ight index,HW I)、左室质量指数(left ventricu-larm ass index,LVM I);光镜、透射电镜观察左室心肌细胞形态结构的改变。结果(1)与SHR-C组比较,灌胃4,8周后,各治疗组SBP显著降低(P<0.01);(2)与SHR-C组比较,灌胃8周后,SHR-Y1、SHR-Y2及SHR-X组HW I、LVM I均明显降低(P<0.05或P<0.01);(3)光镜观察发现:SHR-C组心肌纤维增粗或萎缩、断裂或排列紊乱,部分心肌细胞核肥大或固缩;透射电镜观察发现:SHR-C组可见部分心肌细胞核膜皱缩,核染色质凝聚、趋边,偶见核固缩,部分心肌细胞线粒体明显肿胀。与SHR-C组比较,SHR-Y1组上述病变明显减轻;SHR-Y2、SHR-X组病变有所改善;SHR-Y3组病变改善不明显。结论益气活血复方、硝苯地平均能降低SBP、HW I、LVM I,减缓SHR左室肥厚形成、发展过程中左室组织结构的破坏。
Objective To explore the effects of Yiqihuoxue decoction on left ventricular hypertrophy and the morphological structure of myocardial cells in left ventricle of spontaneously hypertensive rats（SHR）. Methods Fifty fourteen-week-old male SHR were divided randomly into 5 groups： SHR-Y1 （treated with high dose of Yiqihuoxue decoction） , SHR-Y2（ treated with moderate dose of Yiqihuoxue decoction） , SHR-Y3 （ treated with low dose of Yiqihuoxue decoction） , SHR-C （untreated） , SHR-X （ treated with nifedipine）. The systolic blood pressure（SBP） was measured before and during treatment every four weeks. After 8 weeks of treatment, all rats were killed. Heart weight index（HWI） , left ventricular mass index（LVMI） were calculated. The morphological and structural changes of myocardial cells in left ventricle of SHRs were observed with light microscope and transmission electron microscope（TEM）. Results （1） After 4 or 8 weeks＇treatment, compared each treatment group with SHR- C,the SBP decreased slgnificantly（P 〈0.01 ）. （2）After 8 weeks＇treatment, compared SHR-Y1, SHR-Y2 and SHR-X with SHR-C respectively, both HWI and LVMI decreased significantly （P 〈 O. 05 or P 〈 O. O1 ）. （3） The results under light microscope were：in SHRC, cardiac muscle fibers were swollen, decreased, disrupted or disorganized arrangement, as well as some hypertrophic nuclear or pyknosis of myocardial cells. The results under TEM were： in some left ventricular tissues obtained from SHR-C group, nuclear membrane shrank and nuclear chromatin condensed and congregated under nuclear membrane like speckle, abutting against the nuclear envelope; and pyknosis was observed by chance. Mitochondria of some myocardial cells were markedly swollen with some disorganized or disrupted mitochondrial cristae which displayed like uneven vacuole. Compared with SHR-C, the above - men- tioned pathological changes of SHR-Y1 were less obvious; the pathological changes ameliorated slightly in SHR-Y2 and SHR-X; but there was no significant improvement in SHR-Y3. Conclusion Yiqihuoxue decoction and nifedipine can reduce the SBP, HWI, LVMI and slow down the damages of left ventricle in SHRs during the process of LVH.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第9期1635-1637,共3页
Lishizhen Medicine and Materia Medica Research
基金
广东省中医药局立项课题(No.1050116)
关键词
益气活血复方
自发性高血压大鼠
左室肥厚
形态结构
Yiqihuoxue decoction
Spontaneously hypertensive rats
Left ventricular hypertrophy
Morphological structure
