摘要
目的研究新疆紫草提取物AEJ-I、AEJ-Ⅱ对小鼠急性四氯化碳(CC l4)性肝损伤的保护作用。方法腹腔注射0.1%CC l4橄榄油溶液制备小鼠急性化学性肝损伤模型,以肝脏系数、脾脏系数,血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST),肝组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)的水平和肝脏病理组织学变化为观测指标,观察AEJ-I、AEJ-Ⅱ对小鼠急性CC l4性肝损伤的影响。结果AEJ-I、AEJ-Ⅱ各剂量组均可显著降低小鼠血清ALT,AST活性(P<0.01);AEJ-I高剂量组能使肝组织中SOD、GSH-Px活性升高、MDA含量下降(P<0.05);AEJ-I低剂量组可以升高肝组织GSH-Px活性(P<0.05),而对肝组织其它指标均无明显影响(P>0.05);AEJ-Ⅱ高剂量组能降低MDA含量(P<0.05),而对肝组织其它指标AEJ-Ⅱ各剂量组影响均不明显(P>0.05);AEJ-I、AEJ-Ⅱ均可不同程度地改善肝脏病理组织性损伤。结论AEJ-I、AEJ-Ⅱ对小鼠急性CC l4性肝损伤具有保护作用。
Objective To investigate the protective effects of Arnebia euchroma （Royle） Johnston extracts （ab. AEJ-I, AEJ-Ⅱ） a- gainst CCl4-induced acute liver injury in mice. Methods The acute liver injury animal model was established in mice by intraperitoneal injection of 0.1% CCl4 olive oil . The coefficient of liver and spleen; alanine aminotransferase （ALT） and aspartate amiontransferase （AST） activities in serum ; superoxide dismutase （SOD） ,glutathione peroxidase （GSH-Px） activities and malondialdehyde （MDA） content in liver homogenate were assayed and hepatic pathological changes were observed, Results AEJ-I and AEJ-Ⅱ significantly decreased the activities of serum ALT and AST in mice （P 〈 0.01 ） ; AEJ-I high dose group increased the activity of liver homogenate SOD ,GSH-Px and decreased the content of MDA （P 〈0, 05） ; AEJ-I low dose group increased the activity of liver homogenate GSH-Px （P 〈 0.05 ） , but it had no marked effect on other indexes of liver homogenate mentioned above （P 〉 0, 05 ） ; AF_J-II high dose group decreased the content of MDA （P ~ 0.05） , but AEJ-II had no marked effect on other indexes of liver homogenate mentioned above（P 〉 0.05） ; AEJ-I and AEJ-Ⅱ improved liver histopathology injuries. Conclusion AEJ- I, AEJ-Ⅱ showed protective effects on acute liver injury induced by CCl4 in mice.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第9期1676-1678,共3页
Lishizhen Medicine and Materia Medica Research
关键词
新疆紫草
急性肝损伤
Arnebia euchroma （Royle） Johnston
Acute liver injury
