摘要
采用兔抗5-羟色胺抗体和SABC免疫细胞化学方法,对日本囊对虾视神经节、脑和胸神经节进行定位研究。结果表明,视神经节内的5-羟色胺免疫阳性细胞分布于视外髓、视内髓和视端髓。前脑前中群,前脑中部前端神经髓质、中央体,中脑嗅叶内侧细胞群,后脑后中群、侧位群和后侧群呈5-羟色胺免疫阳性反应。胸神经节5-羟色胺阳性细胞较少,第4对胸神经节检出1对阳性细胞,第5对胸神经节检出2对阳性细胞,神经髓质不呈阳性反应。5-羟色胺的特异性分布,为日本囊对虾体内的生理功能和甲壳动物腹神经索演化提供了形态学依据。
To investigate the distribution of 5-HT in the central nervous systems of M. japonicus, 5-HT antisera in rabbit and immunocytochemistry method of Strept Avidin-Biotin-Complex were applied to observe 5-HT-immunoreactive neurons and neuropils in optical, brain, and thoracic ganglion. Results showed that： except for lamina ganglionaris （LG） , 5-HT-immunoreactivity were found in medulla externa （ME） , medulla interna （MI） , and medulla terminalis （MT） in optic ganglia. There were a few 5-HT-immunoreactive cells in ME and MI, but much more cells in MT and some small immunoreactive cells aggregated in X organ. Many 5-HT-immunoreactive cells were located in anterior median cluster. The anterior medial protocerebrum neuro- pils （AMPN） and central body also showed 5-HT immunoreactivity in protocerebrum. There were many 5-HT- immunoreactive cells of different sizes distributed in the inner cell cluster lateral to olfactory in the deutocerebrum. Only a few large 5-HT-immunoreactive cells were found in the clusters of posterior median, lateral, and posterior lateral of tritocerebrum. 5-HT-immunoreactive cells were less in the thoracic ganglions. Only one pair 5-HT- immunoreactive cells were located in the fourth thoracic ganglion and two pairs immunoreactive cells in the fifth thoracic ganglion. The neuropils showed no 5-HT immunoreactivity.
The 5-HT immunoreactivity distribution in central systems of M. japonicus provided morphological proofs for its neurophysiological functions and phylogeny of crustacean ventral nerve cords. Further investigation is suggested on exact content and 5-HT mechanics in different physiological states.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2006年第5期412-416,共5页
Oceanologia Et Limnologia Sinica
基金
国家自然科学基金项目资助
30300269号
福建省青年科技人才创新项目资助
2003J018
关键词
日本囊对虾
视神经节
脑
胸神经节
5-羟色胺
免疫细胞化学
Marsupenaeus japonicus, Optic ganglia, Brain, Thoracic ganglion, 5-HT, Immunocyto-chemistry
