摘要
2004年12月～2005年12月间，对南宁市及其周边地区26个商业肉鸭群鸭疫里默氏杆菌（Riemerella anatipestifer）病的发生情况进行了调查。从167羽具有浆膜炎病变的病死鸭中，应用巧克力培养基的分离培养，以及对分离株进行的生化试验，细菌外膜蛋白基因的聚合酶链式反应（PCR）扩增的鉴定，结果分离到74株鸭疫里默氏杆菌；通过玻片凝集试验鉴定，所有分离株均属于血清2型；在鸭疫里默氏杆菌阳性病料中同时分离到23株大肠杆菌，混合感染率高达31％；脑组织的分离率明显高于肝脏组织的；在2～6周龄的发病时间段里，肉鸭对鸭疫里默氏杆菌的感染表现出明显的年龄抵抗力；常用药物的敏感性试验表明大多数分离株对头孢唑啉、头孢啦啶、头孢哌酮表现高度敏感。
The investigation on Riemerella anatipestifer （RA） infection in 26 commercial flocks of meat-type ducks in Nanning area was conducted in the time of December 2004 to December 2005. Totally 74 isolates isolated with chocolate agar from 167 ducks with serositis were identified as RA by the biochemical reaction and the PCR amplifications of the outer membrane gene. Slide agglutination test demonstrated that all the isolates belong to serotype 2 of RA. 23 Escherichia coli （E. coli） were isolated ＇concurrently from the RA positive ducks, and with a 31% co-infection rate. The isolate rate of brain was significant higher than that of liver. A significant age resistance against RA infection was observed during the period of 8 day-old to 42 day-old. Most of the RA isolates were highly sensitive to Cefazolin, Cefradine, and Cefoperazone by the antibacterial sensitivity test with the common antibiotics.
出处
《广西农业生物科学》
CAS
CSCD
2006年第3期210-213,260,共5页
Journal of Guangxi Agricultural and Biological Science
关键词
鸭疫里默氏杆菌
分离与鉴定
血清型
混合感染
药敏试验
Riemerella anatipestifer
isolation antibacterial sensitivity test and identification
serotype
co-infection
