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胃泌素受体放射配基结合分析法的建立 被引量:2

Establishment of radioligand binding assay of gastrin receptors
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摘要 建立了胃泌素受体(GR)的放射配基结合分析法,测定34例胃癌及邻近正常粘膜胃泌素受体含量及亲和力Kd。发现五肽胃泌素(Gs-5)及[Leu15]Gs-17均能作为非标记配基,Gs-5的抑制效果更好。醋酸纤维混合膜(Φ0.45μm)分离结合及游离部分125[Leu15]Gs-17效果好。34例胃癌及其邻近正常粘膜表达GR是一致的。 Radioligand binding assay of gastrin receptor(GR) was established to determine GR content and Kd of gastric cancer and mucosa in specimens of 34 patients. Both of Gs-5 and [len15]Gs-17, as unlabelled ligand, can inhibit the binding of 125I-(leu15)Gs-17to the membrane, and the inhibiting effect of Gs-5 is slightly stronger than that of (len15)Gs-17.Mixed cellulose acetate filters have better effect to separate bound 125I (leu15)Gs-17 from free 125I- (len15)Gs-17 than glass fibre filters (Hongguan 999999).The GR of gastric cancer correlates with that of mucosa in the same region of 34patients.
出处 《核技术》 CAS CSCD 北大核心 1996年第11期653-656,共4页 Nuclear Techniques
关键词 胃泌素受体 放射配基 结合分析 胃癌 Gastrin receptor(GR), Radioligand binding assay, Gastric cancer
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参考文献1

  • 1夏宗勤,实验核医学与核药学,1989年

同被引文献12

  • 1李燕舞,王建华,王汝俊.大鼠胃壁细胞的分离及纯化[J].世界华人消化杂志,2004,12(7):1733-1735. 被引量:6
  • 2聂克 王汝俊 王建华 等.脾虚大鼠胃壁细胞胃泌素受体结合容量及黄芪对其调控作用[J].中药药理与临床,2000,(16):39-40.
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  • 6Dockray GJ.Gastrin and gastric epithelial physiology.J Physiol,1999,518(Pt 2):315~324.
  • 7Delvalle J,Tsunoda Y,Williams JA,et al.Regulation of[Ca^2+]i by secretagogue stimulation of canine gastric parietal cells.Am J Physiol,1992,262(3 Pt 1):G420~G426.
  • 8Chew CS,Brown MR.Release of intracellular Ca^2+ and elevation of inositol trisphosphate by secreagogues in parietal and chief cells isolated from rabbit gastric mucosa.Biochim Biophys Acta,1986,888(1):116~125.
  • 9聂克,王汝俊,王建华.大鼠胃壁细胞的分离方法[J].世界华人消化杂志,1999,7(11):994-995. 被引量:25
  • 10聂克,王汝俊,王建华.大鼠胃壁细胞胃泌素受体的放射配基结合法[J].中国药理学通报,2001,17(6):645-647. 被引量:8

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