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侵染花生的辣椒褪绿病毒S RNA全序列分析 被引量:5

Complete Sequence Analysis of Genomic S RNA of Capsicum chlorosis Virus Infecting Peanuts in China
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摘要 番茄斑萎病毒属(Tospovirus)是布尼亚病毒科(Bunyaviridae)植物病毒组成的一个属,病毒粒子为球状,直径80~110nm,粒体外层由一层脂质包裹。基因组属于负单链RNA,由三个片段组成,分别被称为LRNA、MRNA、和SRNA。LRNA为负链、含单个开放阅读框架(ORF),MRNA和SRNA均为双义RNA,有2个ORF,反向相连。SRNA大小约3kb,编码分子量为54kDa的非结构蛋白(NSs)和分子量为29kDa的核衣壳蛋白(N)。目前该属已正式定名的病毒有16种,分布在世界各地。Chen等根据病毒N基因氨基酸序列同源性,将16种病毒划分为TSwV、WSMoV、IYSV3个主要血清组和INSV、PYSV、PCFV、MYSV单一病毒构成的4个血清型。其中,属于WSMoV血清组的有西瓜银色斑驳病毒(Watermelon silver mottle virus,WSMoV)、花生芽坏死病毒(Peanut bud necrosis virus,PBNV)、辣椒褪绿病毒(Capsicum chlorosis virus,CaCV)、西瓜芽坏死病毒(Watermelon bud necrosis virus,WBNV)和兰花褪绿斑病毒(Calla lily chlorotic spotvirus,CCSV). A new tospovirus was discovered in infected peanut plants based on complete sequencing and analysis of its genomic S RNA. It was characterized as a new strain of Capsicum chlorosis virus (CaCV) and was named China peanut (CP) strain. The total sequence of S RNA of CaCV-CP was 3399 nucleotide (nt) and contained two open reading frames (ORFs) with an ambisense arrangement. ORF1 was 1320nt encoding a 49.9 kDa nonstructural (NSs) protein. ORF2 is 828nt encoding a 30.7 kDa nucleocapsid (N) protein. The N gene of CaCV-CP shared 84.7%-86.4% and 92.4%-93.1% identity with that of CaCV strains from Thailand and Australia at the nucleotide and amino acid levels, respectively. This is the first report of complete sequence of CaCV S RNA and occurrence of CaCV in China.
出处 《中国病毒学》 CSCD 2006年第5期506-509,共4页 Virologica Sinica
基金 国家自然科学基金(30170606)
关键词 辣椒褪绿病毒 株系 SRNA 序列分析 Capsicum chlorosis virus Strain S RNA Sequence analysis
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