摘要
目的探讨血液透析患者脂蛋白(a)[Lp(a)]代谢紊乱机制,为寻找有效控制Lp(a)紊乱的方法提供理论基础。方法收集53例透析患者、42例体检健康者空腹血清,AEROSET-2000全自动生化分析仪测定血清Lp(a)。酶联免疫吸附法(ELISA)检测氧化低密度脂蛋白(ox-LDL)、白介素-1β(IL-1β)、白介素-6(IL-6)、白介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)。结果透析组血清Lp(a)水平均显著高于对照组;透析组血清炎症相关因子IL-6、IL-8、TNF-α均显著高于对照组,IL-1β水平无显著性差异;相关性分析显示IL-6、IL-8与Lp(a)水平显著正相关。透析患者血清ox-LDL含量与对照组比差异显著,且与Lp(a)呈非常显著正相关(P<0.01)。结论透析患者处于炎症、氧化应激状态可能是Lp(a)代谢紊乱且不易控制的原因之一。
Objective To clarify the mechanism of high levelin hemodialysis patients Lp （a） in hemodialysis patients. Methods The study was conducted on a sample of 53 chronic renal failure patients （males： 37, females： 16, age 23-75 years） who had receired hemodialysis treatment above one year. Fourty-two samples from healthy physical examination were as controls. Lp（a） level were detected by AEROSET-2000 biochemistry auto-analyzer. The quantifications of 0x-LDL, IL-l beta, IL-6, IL-8 and TNF-alpha were performed with double-antibody ELISA. Results The serum Lp（a） levels were remarkably higher in CRF patients than in the control subjects （P〈0.05）. The serum ox-LDL IL-6 and IL-8, TNF- α levels in hemodialysis patients were remarkably higher than in the control subjects, but there were no significant difference of IL-1 β level between the two groups. There were significant correlations between Lp（a） and IL-6, IL-8 and ox-LDL. Conclusions Lp（a） has comelation with inflammation and oxidative stress. It maybe one of the reasons of in creased and disordered Lp（a） level in hemodialysis patients.
出处
《中国血液净化》
2006年第9期661-662,共2页
Chinese Journal of Blood Purification
基金
辽宁省自然科学基金资助(编号:20042055)
