摘要
目的观察氟对体外培养软骨细胞内质网的影响及超氧化物歧化酶(sOD)对氟的拮抗作用。方法取大鼠肋软骨进行体外培养,在培养液中加入不同浓度的NaF或相应浓度NaF+SOD,用UV754分光光度计测定软骨细胞培养基中H_2O_2和NO。运用激光共聚焦显微镜观察大鼠软骨细胞内质网的改变。结果与对照组比较,实验各组培养液中H_2O_2的水平均显著升高,差异有统计学意义(P＜0．05),且随着含氟量的增高,H_2O_2的水平也随之升高。加入SOD后,各实验组H_2O_2的水平逐渐减少,与相应不加SOD组比较,以80μmol/L NaF+SOD、160μmol/L NaF+SOD组降低为显著(P＜0．05)。与相应不加SOD组比较,NO的水平降低,只见于40μmol/L NaF+SOD组,差异有统计学意义(P＜0．05)。内质网改变在40、80、160μmol/L NaF条件下,内质网都表现荧光强度减弱；40μmol/L NaF+SOD组与40μmoL/L NaF组相比,内质网荧光强度改变不明显。80μmol/L NaF+SOD组与80μmol/L NaF组、160μmol/L NaF+SOD组与160μmol/L NaF组相比,内质网荧光强度明显增加。结论(1)不同氟水平对培养软骨细胞内质网有影响。(2)SOD对氟培养软骨细胞内质网的损伤有拮抗作用,对高氟损伤的拮抗作用更明显。(3)激光共聚焦显微镜对培养软骨细胞内质网的定量分析,能更准确的反映氟中毒时软骨细胞的功能状态。
Objective To study the influence of fluoride on the endoplasmic reticulum of cultured chondrocytes and antagonizing effect of superoxide dismutase （SOD）. Methods The chondrocytes from the Wistar rat were cultured in vitro for 7 days. Various concentrations of fluoride with or without SOD were added to the cultured medium. Endoplasmic reticulum was observed using laser scanning confocal microscope （LSCM）. Results The average optical density（AOD） of endoplasmic reticulum was obviously decreased respectively cultured with 40, 80 and 160 μmoL/L of NaF, while it was obviously increased when 80 μmol/L and 160 μmol/L of NaF was added with SOD. Conclusions （ 1 ）Endoplasmic reticulum is influenced by various concentration of fluoride. （2）SOD has antagonistic effect on the damage caused by fluoride, especially by high level of fluoride. （3）Quantitative analysis on the endoplasmic reticulum of chondrocytes with laser scanning confocal microscope accurately identifies the functioning state of chondrocytes under fluorosis.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2006年第5期507-510,共4页
Chinese Jouranl of Endemiology
