摘要
目的:研究了壳聚糖膜中血红蛋白分子对青蒿素的催化还原作用。方法:根据血红蛋白在壳聚糖膜的微环境中有效的电子转移和良好的稳定性,将血红蛋白和壳聚糖的混合溶液涂在玻碳电极表面,形成血红蛋白-壳聚糖薄膜,用于青蒿素的电化学催化还原研究。结果:在pH 7.1缓冲液中,血红蛋白分子能诱导青蒿素过氧桥键断裂,从而使青蒿素-1.04V左右的还原峰正移至-0.48V,青蒿素阴极过电位降低了520mV。结论:该新峰为青蒿素与血红蛋白复合物的还原峰,对更深入阐明青蒿素药物的药理作用机制具有重要意义。
Objective： The interaction between artemisinin and hemoglobin was investigated by electrochemical methods. Method： With the stability of hemoglobin in the microenvironment of chitosan film, the mixture of chitosan and hemoglobin solutions were casted onto the surface of glassy carbon electrode, and formed a fiat of thin film. Result： It was showed that artemisinin can be reduced by hemin in hemoglobin and its original reduction peak at-1.04 V disappeared and another peak appeared at - 0.48 V in pH 7. 1 buffer solution. The cathodic overpotential of artemisinin globin in chitosan decreased by 520 mV . Conclusion：The catalytic reduction mechanism of artermisinin by hemofilm have active effect.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第9期1254-1257,共4页
Chinese Journal of Pharmaceutical Analysis
基金
国家自然科学基金(No.59932050)
关键词
壳聚糖
血红蛋白
青蒿素
催化还原
chitosan
hemoglobin
artemisinin
catalytic reduction
