摘要
目的:探讨周围神经嵌压性损害早期c-fos基因的表达及Ca2+通道阻滞剂(CCB)对周围神经嵌压性损害保护作用的机制。方法:将80只SD大鼠按体重随机分层分组制作坐骨神经嵌压性损害的动物模型,取嵌压后第1、4周的大鼠坐骨神经,采用免疫组织化学结合电生理学的方法测定c-fos基因的表达及坐骨神经传导速度,并将两者结果进行对比,运用正常对照组、模型组、CCB高剂量和CCB低剂量组进行对照统计。结果:嵌压大鼠坐骨神经后,大鼠坐骨神经c-fos基因的表达第1周时显著增加(P<0.01),第4周时趋于正常;CCB可减少1周时大鼠背根神经节细胞c-fos基因的表达,高剂量组尤为显著(P<0.01);第4周时CCB组坐骨神经的传导速度虽较正常对照组及假手术组慢(P<0.01),但较模型组快,高剂量组显著(P<0.05)。结论:周围神经嵌压性损害早期c-fos基因表达增加,并使神经功能受损;CCB则可通过阻断Ca2+内流过程而减少早期神经细胞c-fos基因的表达,从而对周围神经嵌压性损害具有保护作用。
Objective：To investigate the expression of c-los gene in crush lesion of peripheral nerve in early stage, and the protective mechanism of calcium channel blocker （CCB）. Methods：Sciatic nerve was crushed by pincers to establish the model of sciatic nerve crush lesion in rats, The expression of c-fos gene and conductive rate of sciatic nerve were quantitated by inlnlunohistochemistrical and electrophysiological techniques. SD rats were divided into normal group,pseudo-operation group, placebo control group, CCB （high-dose or low-dose Flunarizine） group at randonl, Results：The expression of c-fos gene in sciatic nerve was increased significantly on the first week after sciatic nerve crush lesion （P 〈 0.01 ） ,and it was decreased by CCB significantly in the high-dose group （P 〈 0.01 ）, Conductive rate of sciatic nerve on the fourth week after sciatic nerve crush lesion was slower in CCB groups than that in normal group and pseudo-operation group, but higher than that in placebo control group （P 〈 0.05）, It was decreased by CCB in a dose-dependent manner（P 〈 0.01 ）. Conclusion： The expression of c-fos gene is increased significantly, which causes injury of nerve function in early stage of peripheral nerve crush lesion. CCB could decrease c-los gene enpression by blocking Ca^2＋ internal flow. It shows that CCB has the protective action against peripheral nerve crush lesion.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第10期898-901,F0002,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省自然科学基金(BK2001116)资助项目
