摘要
目的:建立大鼠血浆及组织(心、肝、脾、肺、肾及淋巴结)中米托蒽醌(MTO)的快速萃取法和 RP-HPLC 检测法,研究米托蒽醌白蛋白纳米粒(MTO-BSA-NP)在大鼠体内的组织分布和淋巴结靶向性。方法:采用甲酸为酸化剂、甲醇为润湿剂、20%三氯乙酸为蛋白沉淀剂、氯仿为油脂溶解剂一步沉淀萃取 MTO。RP-HPLC 法检测 MTO,色谱柱为 C_(18)(300mm×6mm,5μm),流动相为甲醇-0.16mol·L^(-1)甲酸铵缓冲液(pH2.7)(48:52),进样量10μL,流速1.0mL·min,检测波长658nm,外标法峰面积定量。结果:MTO 与内源性物质分离良好。血浆及心、肺、肾和淋巴结匀浆中,MTO 浓度在50～2997ng·mL^(-1)范围内;肝和脾匀浆中,MTO 浓度在100～2997ng·mL^(-1)范围内,峰面积与 MTO 浓度线性关系良好(r>0.999)。MTO 从血浆中萃取同收率为98.5%,组织中的萃取回收率均在52.0%以上。方法回收率高,日内和日间精密度均小于10%,样品稳定性良好。测定结果表明 MTO-BSA-NP 在淋巴结的摄取率是米托蒽醌注射液(MTO-Soln)的3.36倍,但在其他组织的分布无显著性差异。结论:本文建立的一步沉淀萃取法和 RP-HPLC 法,简便、快速、可靠,可用于 MTO-BSA-NP 在大鼠的体内分布和淋巴结靶向性研究。MTO-BSA-NP 较 MTO-Soln 淋巴结靶向性显著。
Objective：To develop a simple,rapid RP - HPLC method for determination of mitoxantrone（MTO） in rat plasma and different tissues （lymph nodes, liver, spleen, heart, and kidneys） after sc injection of mitoxantrone loaded albumin nanoparticles （MTO- BSA- NP）. Methods：One- step protein precipitating procedure was used to prepare samples by adding formic acid and methanol to increase the mitoxantrone extraction recovery, 20% CCl3COOH to precipitate protein and chloroform to dissolve lipids. Assay of mitoxantrone was carried out using C18 reversed phase HPLC column（300 mm × 6 mm,5 μm） , with the mobile phase of methanol -0. 16 mol · L^-1 ammonium formate buffer（pH 2.7） （48： 52） ,injection volumn of 10 μL and flow rate of 1.0 mL · min^-1 at 658 nm by external standard method. Results：Mitoxantrone can be baseline separated with other substances. In plasma,heart, kidney and lymph node,the standard curves were linear from 50 to 2997 ng · mL^-1. In liver and spleen, the standard curves were linear from 100 to 2997 ng · mL^-1. The absolute recovery of mitoxantrone from the plasma was 98.5% and other homogenates were all over 52.0%. The within- day and between- day precision were all below 10%. The samples were stable during the storage, extraction and assay process. AUC for MTO -BSA -NP in lymph node was 3.36 folds higher than that for mitoxantrone solution （ MTO - Soln ）. Conclusion ： The method described here is suitable for the study of mitoxantrone distribution and pharmacokinetics in rat plasma and different tissues after sc injection of MTO - BSA - NP. MTO - BSA - NP significantly facilitated MTO to concentrate in lymph node than that of MTO - Soln.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第8期1043-1049,共7页
Chinese Journal of Pharmaceutical Analysis
关键词
米托蒽醌
白蛋白纳米粒
体内分布
淋巴结靶向性
高效液相色谱法
mitoxantrone
mitoxantrone loaded albumin nanoparticles
tissue distribution
lymph node targeting
RP- HPLC
