摘要
重组海藻糖合成酶工程菌的分批补料发酵中,用氨水调节培养液的pH为6.78～6.80,初糖耗尽后按培养液的pH从6.80起每升高0.01即开始补充葡萄糖至0.7g·L-1。据此补料策略可将发酵过程的乙酸积累控制在6.26g·L-1以下;补料发酵12h后,即获得细胞干重为51.5g·L-1的细胞高密度,细胞密度是分批培养的7.5倍;诱导后重组海藻糖合成酶表达率为15.2%,单位体积内的表达量是分批培养的4.5倍。
pH-stat fed-batch fermentation of recombinant Escherichia coli was carried out in 40L biostat fermentator. Feeding solution was added when the pH rose to a value greater than its set point by 0.01. After 12h feeding, the dry cell weight reached as high as 51.5g·L^-1 and the acetic acid was only 6.26g·L^-1 When ceils were induced by IPTG, the expression of trehalose synthase was accounted for 15.2% of total soluble proteins.
出处
《食品工业科技》
CAS
CSCD
北大核心
2006年第8期125-128,共4页
Science and Technology of Food Industry
基金
国家"863"计划(AA214171)
