摘要
目的:通过检测MRP蛋白的表达,探讨人涎腺腺样囊性癌细胞产生多药耐药的机制。方法:用浓度为1μg/ml的顺铂作用于腺样囊性癌细胞,每次作用时间48 h,经6个月后形成在该药物浓度下生长良好的耐顺铂细胞。运用免疫组化、Western-blot法检测SACC和SACC/DDP细胞株中MRP蛋白的表达,RT-PCR检测SACC和SACC/DDP的MRP mRNA的表达。结果:诱导形成的顺铂作用环境下生长稳定的细胞株SACC/DDP,其MRP蛋白和MRP mRNA表达水平明显高于母本细胞SACC(P<0.01)。结论:SACC/DDP细胞的细胞浆中以及细胞膜上MRP蛋白的表达率很高,可能是涎腺腺样囊性癌细胞产生多药耐药的机制所在。
Objective：To study the mechanism of the multidrug resistance（MDR） of human SACC/DDP cell line by detecting the expression of multidrug resistance - associated protein（MRP）. Methods. SACC cell line was exposed to 1μg/ml of DDP for 48 h every month and the cell line of SACC/DDP was established six months later. Irnmunohistochemical SP and Western - blot were taken to examine the expression of MRP in SACC and SACC/DDP and RT - PCR was taken to test the expression of MRP mRNA in SACC and SACC/DDP. Results：The expression of MRP and MRP rnRNA in SACC/DDP was apparently higher than that in SACC（P〈0. 01）. Conclusion：The expression of MRP in SACC/DDP was higher than that in SAC.C, which maybe the mechanism of MDR in human SACC/DDP cell line.
出处
《西南国防医药》
CAS
2006年第5期470-473,共4页
Medical Journal of National Defending Forces in Southwest China
关键词
人涎腺
腺样囊性癌
多药耐药
MRP蛋白
salivary, salivary adenoid cystic carcinoma, multidrug resistance, multidrug resistance - associated protein
