摘要
以毕克齐长山药节间茎段为外植体进行组织培养，探讨不同激素、N素处理对不定芽的形成及继代增殖的影响。结果表明：0．1mg·L^-1以下低浓度的6-BA对不定芽的形成有促进作用，其中MS＋6-BA0．1mg·L^-1＋NAA0．01mg·L^-1为适宜培养基，不定芽的形成率达75％；不定芽增殖适宜培养基为MS＋6-BA2．0mg·L^-1＋NAA0．5mg·L^-1，平均每个外植体再生芽数7．6个；培养基MS＋IBA0．5mg·L^-1生根率可达100％。
Bikeqi long yam is a kind of local vegetable in Inner Mongolia. Its internodal tissue culture and propagation were carried on. The most effective for the introduction of adventitious buds was the MS media containing 6 - BA 0.1 mg · L^- 1 and NAA 0.01 mg · L^-1. The shooting rate was 75 %. The optimum propagation cultural media was MS ＋ 6 - BA 2.0 mg· L ^- 1 ＋ NAA 0.5 mg· L^- 1. The mean number of shoots was 7.6. MS ＋ IBA 0.5 mg · L ^- 1 was the optimum media for rooting. The rate was 100 %.
出处
《中国蔬菜》
北大核心
2006年第B10期47-49,共3页
China Vegetables
关键词
毕克齐长山药
节间组织培养
增殖
Bikeqi long yam,Internodal tissue culture, Propagation
