摘要
研究海洋生态系统在调节全球变化中的作用,采用生态毒理学的方法研究了UV-B辐射增强对2种海洋微藻膜的损伤及其差异性。结果表明,随着UV-B辐射剂量的增加,叉鞭金藻(Dicrateriasp.)和三角褐指藻(Phaeodactylum tricornu-tum)的光合速率降低。同时,膜相对透性增大,细胞内H2O2含量上升,微粒体膜中磷脂减少,游离脂肪酸增加。表明UV-B辐射增强使2种微藻的膜受到了严重伤害,而且膜伤害的加剧与活性氧的积累和由此引发的膜脂脱酯化有关。2种微藻细胞匀浆中丙二醛(MDA)含量无显著变化,而微粒体膜的MDA含量随着UV-B辐射剂量的加大显著提高。这表明UV-B辐射增强对2种海洋微藻膜的损伤可能是由膜脂过氧化和脱酯化作用共同引起的。
The membrane damage and its differences caused by UV-B radiation enhancement to two species of marine microalgae were studied using ecotoxicology methods. The results showed that the membrane relative permeability and H2O2 content increased, and the photosynthesis rate decreased in Dicrateria sp. and Phaeodactylurn tricornutum with the UV-B radiation enhancement. Meanwhile, phospholipid content decreased and free fatty acid content promoted in microsomal membrane. These indicated that the membrane damage in the two species of marine microalgae was seriously induced by UV-B radiation enhancement, and the exacerbation of membrane damage was really relative to the accumulation of active oxygens which initiated deesterification. Although malondiadehyde（MDA） content in homogenate of two species of marine microalgae did not Show significant change, it exhibited obvious increment in microsomal membrane. So it was concluded that the membrane damage under UV-B radiation enhancement was caused by membrane lipid peroxidation in combination with membrane lipid deesterification.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第5期763-766,共4页
Periodical of Ocean University of China
基金
国家自然科学基金项目(30270258)
新世纪优秀人才支持计划奖励基金项目(NCET-05-0597)资助
关键词
UV-B辐射增强
膜脂过氧化
脱酯化
海洋微藻
UV-B radiation enhancement
membrane lipid peroxidation
deesterification
marine microalgae
