摘要
目的探讨端粒酶活性在肺癌患者诱导痰中表达的临床意义。方法采用TRAP-PCR-ELISA法对30例肺癌患者诱导痰、自然痰及15例肺部良性病变者诱导痰中端粒酶活性进行测定。结果肺癌患者的诱导痰、自然痰以及非肺癌患者诱导痰中的端粒酶阳性率分别为80.0%、56.7%、13.3%;OD值分别为(0.52±0.27)、(0.35±0.25)、(0.09±0.11)。肺癌诱导痰组端粒酶的阳性率及OD值均高于自然痰组及良性对照组,差异有显著性意义(P<0.01)。10例小细胞肺癌(SCLC)化疗后诱导痰中端粒酶OD值明显下降,与化疗前比较差异有显著性意义(P<0.01)。结论诱导痰中端粒酶活性的检测有助于肺癌的诊治及其与肺部良性疾病的鉴别诊断,且优于自然痰。
Objective To investigate the clinical significance of detecting telomerase activity in induced sputum in patients with lung cancer. Methods The telomerase activities in induced sputa and spontaneous sputa from 30 patients with lung cancer and 15 patients with benign lung disease were assayed with TRAP - PCR - ELISA. Results The positive rates of telomerase activity in induced sputum, spontaneous sputum from lung cancer patients, and induced sputum from benign lung disease patients were respectively 80. 0%, 56. 7% and 13.3% ; and the mean OD values were respectively （0. 52 ± 0. 27 ）, （0. 35 ± 0. 25 ） and （0. 09 ± 0. 11 ）. The positive rate and OD value in induced sputum from patients with lung cancer was statistically significantly higher than those in spontaneous sputum and control groups （ P 〈0. 01 ）. After chemotherapy, the mean OD value of telomerase activity in induced sputa of 10 cases of small cell lung cancer （SCLC） was decreased obviously, with a significant difference （ P 〈0.01 ）. Conclusion The detection of telomerase activity in induced sputum may be helpful to the diagnosis and therapy of lung cancer, and the differential diagnosis with other benign lung diseases; and it is better than that in spontaneous sputum.
出处
《中国全科医学》
CAS
CSCD
2006年第19期1608-1610,共3页
Chinese General Practice
基金
江西省卫生厅资助课题(041139)
关键词
肺癌
诱导痰
端粒
末端转移酶
诊断
Lung neoplasms
Induced sputum
Telomerase
Diagnosis
